Resolution and identification of iron-containing antigens in membrane vesicles from Escherichia coli.
Resolution and identification of iron-containing antigens in membrane vesicles from Escherichia coli.
复制标题
大肠杆菌膜囊泡中含铁抗原的分离和鉴定。
DOI：
10.1021/bi00544a032
复制
发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
H. Kaback
中科院分区：
文献类型：
--
作者：
P. Owen;G. Kaczorowski;H. Kaback
在大肠杆菌ML 308 - 225制备的膜囊泡中存在的含铁抗原通过交叉免疫电泳分析，随后在59 Fe存在下生物体生长。七个离散的抗原（或抗原复合物）检测放射自显影，和六个主要含有非血红素铁。基于这些酶的活性染色，将三种免疫沉淀物阳性鉴定为NADH脱氢酶（EC www.example.com）、NADPH脱氢酶（EC www.example.com）和谷氨酸脱氢酶（EC www.example.com）。含有非血红素铁的另外两种免疫原对应于Owen & Kaback的交叉免疫电泳参考模式中的第22和37号抗原[Owen，P.，& Kapack，H. R.等人（1978）Proc. Acad. sci. U.S.A. 75，3148]。此外，与Braun脂蛋白相对应的免疫沉淀物含有紧密结合的铁。
Iron-containing antigens present in membrane vesicles prepared from Escherichia coli ML 308-225 were analyzed by crossed immunoelectrophoresis following growth of the organism in the presence of 59Fe. Seven discrete antigens (or antigen complexes) are detected by autoradiography, and six contain primarily nonheme iron. Three immunoprecipitates are positively identified as NADH dehydrogenase (EC 1.6.99.3), NADPH dehydrogenase (EC 1.6.99.1), and glutamate dehydrogenase (EC 1.4.1.4) based on activity stains for these enzymes. Two other immunogens containing nonheme iron correspond to antigens no. 22 and 37 in the crossed immunoelectrophoresis reference pattern of Owen & Kaback [Owen, P., & Kaback, H. R. (1978) Proc. Natl. Acad. Sci. U.S.A. 75, 3148]. In addition, the immunoprecipitate corresponding to Braun's lipoprotein contains tightly bound iron.