GENETIC CONSTRUCTION, EXPRESSION, AND MELANOMA-SELECTIVE CYTOTOXICITY OF A DIPHTHERIA TOXIN-RELATED ALPHA-MELANOCYTE-STIMULATING HORMONE FUSION PROTEIN
GENETIC CONSTRUCTION, EXPRESSION, AND MELANOMA-SELECTIVE CYTOTOXICITY OF A DIPHTHERIA TOXIN-RELATED ALPHA-MELANOCYTE-STIMULATING HORMONE FUSION PROTEIN
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DOI：
10.1073/pnas.83.21.8258
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发表时间：
1986-11-01
影响因子：
11.1
通讯作者：
NAGLE, S
中科院分区：
文献类型：
--
作者：
MURPHY, JR;BISHAI, W;NAGLE, S
白喉毒素的结构基因tox通过引入编码独特Pst I限制性内切核酸酶位点的寡核苷酸接头和编码α-寡核苷酸的合成寡核苷酸而在其Sph I位点被修饰。促黑素细胞激素（α- MSH）。所得的融合基因指导白喉毒素相关的α- MSH杂合蛋白，其中白喉毒素受体结合结构域已被α- MSH序列。该嵌合毒素已从重组大肠杆菌K-12的周质提取物中部分纯化，并已发现对α-葡萄球菌有选择性毒性。MSH受体阳性的人恶性黑色素瘤NEL-M1细胞的体外研究
The structural gene for diphtheria toxin, tox, has been modified at its Sph I site by the introduction of an oligonucleotide linker encoding a unique Pst I restriction endonuclease site and a synthetic oligonucleotide encoding .alpha.-melanocyte-stimulating hormone (.alpha.-MSH). The resulting fusion gene directs the expression of a diphtheria toxin-related .alpha.-MSH hybrid protein in which the diphtheria toxin receptor-binding domain has been replaced with .alpha.-MSH sequences. The chimeric toxin has been partially purified from periplasmic extracts of recombinant Escherichia coli K-12 and has been found to be selectively toxic for .alpha.-MSH receptor-positive human malignant melanoma NEL-M1 cells in vitro.