Milk fat globule membrane and its component phosphatidylcholine induce adipose browning both in vivo and in vitro
Milk fat globule membrane and its component phosphatidylcholine induce adipose browning both in vivo and in vitro
复制标题
乳脂肪球膜及其成分磷脂酰胆碱在体内外诱导脂肪褐变
DOI：
10.1016/j.jnutbio.2020.108372
复制
发表时间：
2020-07-01
影响因子：
5.6
通讯作者：
Mao, Xueying
中科院分区：
文献类型：
--
作者：
Li, Tiange;Du, Min;Mao, Xueying
The functional induction of brown-like adipocytes in white adipose tissue (WAT) provides a defense against obesity. The aim of this study was to analyze the effects of milk fat globule membrane (MFGM) and its component phosphatidylcholine (PC) on the brown remodeling of WAT. Male C57BL/6 J mice were fed a high-fat diet (HFD) for 8 weeks and then fed HFD for another 8 weeks with MFGM. In vitro studies were performed in C3H10T1/2 pluripotent stem cells, 3T3-L1 pre-adipocytes and differentiated inguinal WAT stromal vascular cells (SVCs) to determine the role of MFGM and PC on the formation of brown-like adipocytes. MFGM decreased fasting glucose and serum insulin levels in HFD-fed mice. MFGM improved glucose tolerance and insulin sensitivity, and induced browning of inguinal WAT. MFGM and its component PC stimulated transformation of brown-like adipocytes in C3H10T1/2 pluripotent stem cells, 3T3-L1 adipocytes and SVCs by increasing the protein expression of UCP1, PGC-1 alpha, PRDM16 as well as the mRNA expression of other thermogenic genes and beige cell markers. MFGM and PC also increased mitochondrial DNA (mtDNA) copy number, mitochondrial density and oxygen consumption rate and up-regulated the mRNA expression of mitochondria-biogenesis-related genes in vitro. PPARa inhibitor GW6471 treatment or knockdown of PPAR alpha using lentivirus-expressing shRNA inhibited the PC-induced increase in the protein expression of UCP1, PGC-1 alpha and PRDM16 in C3H10T1/2 pluripotent stem cells and 313-L1 adipocytes, indicating the potential role of PPAR alpha in PC-mediated brown-like adipocyte formation. In conclusion, MFGM and milk PC induced adipose browning, which has major protective effects against obesity and metabolic dysfunction. (C) 2020 Elsevier Inc. All rights reserved.
