IDENTIFICATION OF A NOVEL RICKETTSIAL INFECTION IN A PATIENT DIAGNOSED WITH MURINE TYPHUS
IDENTIFICATION OF A NOVEL RICKETTSIAL INFECTION IN A PATIENT DIAGNOSED WITH MURINE TYPHUS
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DOI：
10.1128/jcm.32.4.949-954.1994
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发表时间：
1994-04-01
影响因子：
9.4
通讯作者：
AZAD, AF
中科院分区：
文献类型：
--
作者：
SCHRIEFER, ME;SACCI, JB;AZAD, AF
在美国的几个鼠伤寒疫源地内鉴定出ELB病原体感染的跳蚤和啮齿动物，促使在人鼠伤寒患者中对该病原体进行回顾性调查。这种病原体是最近描述的立克次体，用目前可用的血清学试剂无法与伤寒立克次体区分。分子分析的17 kDa抗原基因和柠檬酸合酶基因区分这种细菌从其他斑疹伤寒组和斑点热组立克次体。目前对其16S核糖体DNA基因的测序结果表明，其与R.伤寒沙门氏菌感染率为99.5%。立克次氏体通过限制性片段长度多态性分析和立克次体特异性PCR产物的Southern杂交分析，5份受检患者血液样本中有1份被ELB感染，而R.其余样本则证实感染伤寒。这是第一次报告观察到人类感染的ELB代理，并强调了实用的PCR促进诊断和区分这些密切相关的立克次体感染。
Identification of ELB agent-infected fleas and rodents within several foci of murine typhus in the United States has prompted a retrospective investigation for this agent among human murine typhus patients. This agent is a recently described rickettsia which is indistinguishable from Rickettsia typhi with currently available serologic reagents. Molecular analysis of the 17-kDa antigen gene and the citrate synthase gene has discriminated this bacterium from other typhus group and spotted fever group rickettsiae. Current sequencing of its 16S ribosomal DNA gene indicates a homology of 98.5% with R. typhi and 99.5% with R. rickettsii. Through a combination of restriction fragment length polymorphism and Southern hybridization analysis of rickettsia-specific PCR products, one of five tested patient blood samples was shown to be infected with ELB while R. typhi infections were confirmed in the remaining samples. This is the first reported observation of a human infection by the ELB agent and underscores the utility of PCR-facilitated diagnosis and discrimination of these closely related rickettsial infections.