Effects of C60 Fullerene on Cell Differentiation with EL-M3 and ES-R1-EGFP B2/EGFP Cell Lines
Effects of C60 Fullerene on Cell Differentiation with EL-M3 and ES-R1-EGFP B2/EGFP Cell Lines
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DOI：
10.11344/nano.6.78
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
K. Imai;T. Nishikawa;Tomoharu Okamura;A. Tanaka;K. Suese;Y. Honda;Tsubasa Shirai;Fumiya Ogawa;H. Sawai;F. Watari
中科院分区：
文献类型：
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作者：
K. Imai;T. Nishikawa;Tomoharu Okamura;A. Tanaka;K. Suese;Y. Honda;Tsubasa Shirai;Fumiya Ogawa;H. Sawai;F. Watari
纳米材料对人类生殖和发育的影响仍不清楚。因此，应检查其胚胎毒性，以确保下一代的生物安全性。在本研究中，使用需要饲养细胞的EL-M3和ES-R1-EGFP B2/EGFP细胞系，而不是用于体外胚胎毒性试验EST方法的ES-D3细胞，研究了C60富勒烯对细胞分化的影响。因此，C60富勒烯对两种细胞系的细胞分化的影响以浓度依赖性方式增加，表明没有严重的发育毒性。C60富勒烯的发育毒性应进行研究，以应用于新药。
The effects of nanomaterials on human reproduction and development remain unclear. Thus, their embryotoxicity should be examined to ensure the biological safety of the next generation. In the present study, the effects of C60 fullerene on cell differentiation were investigated using EL-M3 and ES-R1-EGFP B2/EGFP cell lines that require feeder cells, instead of the ES-D3 cells used for the EST method, an in vitro embryotoxicity test. As a result, the effects of C60 fullerene on cell differentiation increased in a concentration-dependent manner for both cell lines, demonstrating the absence of severe developmental toxicity. The developmental toxicity of C60 fullerene should be investigated for applications to new drugs.