DIETARY OMEGA-3-FATTY-ACIDS DECREASE MORTALITY AND KUPFFER CELL PROSTAGLANDIN-E2 PRODUCTION IN A RAT MODEL OF CHRONIC SEPSIS
DIETARY OMEGA-3-FATTY-ACIDS DECREASE MORTALITY AND KUPFFER CELL PROSTAGLANDIN-E2 PRODUCTION IN A RAT MODEL OF CHRONIC SEPSIS
复制标题
DOI：
10.1097/00005373-199106000-00006
复制
发表时间：
1991-06-01
影响因子：
--
通讯作者：
CERRA, FB
中科院分区：
文献类型：
--
作者：
BARTON, RG;WELLS, CL;CERRA, FB
我们测试了一种假设，即在慢性脓毒症大鼠模型中，用ω-3脂肪替代膳食ω-6脂肪会降低死亡率并减少枯否细胞前列腺素E2（PGE 2）的产生。通过胃造口术给大鼠喂食含15%卡路里的等氮、等热量饮食12天，所述饮食为红花油（omega-6）或鲱鱼油（omega-3）和红花油的10：1混合物。喂食5天后，动物接受了具有确定细菌含量的腹腔内脓肿。在剖腹手术后第6天处死存活者，同时进行肝脏灌注和蛋白酶肝脏消化以分离枯否细胞。在细胞培养18小时后和用0 LPS、10 ng/ml LPS和10 μ g/LPS刺激后，通过放射免疫测定法测量枯否细胞PGE 2的产生。与食用红花油的动物相比，食用鲱鱼油的动物死亡率降低（16% vs. 35%）。与饲喂红花油的动物相比，饲喂鲱鱼油的动物在18小时时（354 +/- 54对570 +/- 95 pg/0.1 ml; p = 0.09）和用10 μ g/ml LPS刺激后（140 +/- 41对288 +/- 45 pg/0.1 ml; p = 0.03）枯否细胞PGE 2产生减少。
We tested the hypothesis that substitution of omega-3 fat for dietary omega-6 fat would reduce mortality and decrease Kupffer cell prostaglandin E2 (PGE2) production in a rat model of chronic sepsis. Rats were fed via gastrostomy for 12 days with isonitrogenous, isocaloric diets containing 15% of calories as either safflower oil (omega-6) or a 10:1 mixture of menhaden oil (omega-3) and safflower oil. After five days of feeding, animals received an intra-abdominal abscess of defined bacterial content. Survivors were killed on post-laparotomy day 6 in conjunction with liver perfusion and protease liver digestion for Kupffer cell isolation. Kupffer cell PGE2 production was measured by radioimmunoassay after 18 hours of cell culture and again after stimulation with 0 LPS, 10 ng/ml LPS, and 10-mu-g/LPS. Mortality was decreased in menhaden oil-fed animals compared with safflower oil-fed animals (16% vs. 35%). Kupffer cell PGE2 production was decreased in menhaden oil-fed animals at 18 hours (354 +/- 54 vs. 570 +/- 95 pg/0.1 ml; p = 0.09) and after stimulation with 10-mu-g/ml LPS (140 +/- 41 vs. 288 +/- 45 pg/0.1 ml; p = 0.03) compared with safflower oil-fed animals.