Detection and Isolation of Preformed Antifungal Compounds from the Peel of Pyrus bretschneideri Rehd. cv. Pingguoli at Different Stages of Maturity
Detection and Isolation of Preformed Antifungal Compounds from the Peel of Pyrus bretschneideri Rehd. cv. Pingguoli at Different Stages of Maturity
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DOI：
10.1111/j.1439-0434.2007.01340.x
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发表时间：
2008-02
影响因子：
1.5
通讯作者：
Yongcai Li;L. An;Y. Ge;Y. Li;Y. Bi
中科院分区：
文献类型：
--
作者：
Yongcai Li;L. An;Y. Ge;Y. Li;Y. Bi
梨皮乙醇-二氯甲烷粗提物。简历。用薄层色谱板分离平果梨，并用交替孢的分生孢子进行生物测定。在膨化期、采收期和冷藏100 d后，不同抑菌区保留系数（R f）差异显著。用气相色谱法和质谱法对抑制区内的化合物进行了分离鉴定。各阶段均存在棕榈酸甲酯、油酸甲酯、亚麻酸甲酯和角鲨烯。这些化学物质的浓度在果皮膨大期最高，随果实发育迅速下降。这些化合物可能是生长果实中主要的抗真菌化合物。收获时和冷藏100 d后，梨皮中邻苯二甲酸酯烷基酯含量较高。从冷藏100天的梨果皮中鉴定出6种邻苯二甲酸酯烷基酯。这些酯类也可能是采后梨的抗真菌化合物。
Ethanol-dichloromethane crude extract from peel of pear (Pyrus bretschneideri Rehd. cv. Pingguoli) was separated by thin layer chromatographic plates and bioassayed with conidia of Alternaria alternata. The inhibition zones differed significantly in retention factor (R f ) at expanding stage, harvest time and after 100 days of cold storage. The compounds in the inhibition zones were isolated and identified with gas chromatography and mass spectroscopy. Palmitate methyl, oleic acid methyl, linolenic acid methyl and squalene were present at all stages. The concentration of these chemicals was the highest in expanding stage fruit peel and decreased rapidly with fruit development. It is suggested that these compounds may be the main antifungal compounds in the growing fruit. The phthalate alkyl esters occurred at relatively higher concentrations in pear peel at harvest and after 100 days of cold storage. Six phthalate alkyl esters were identified from peel of pear fruit after 100 days of cold storage. It is also supposed that these esters may be the antifungal compounds in postharvest pear.