Measurement of human herpesvirus 7 load in peripheral blood and saliva of healthy subjects by quantitative polymerase chain reaction.
Measurement of human herpesvirus 7 load in peripheral blood and saliva of healthy subjects by quantitative polymerase chain reaction.
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通过定量聚合酶链反应测量健康受试者外周血和唾液中的人类疱疹病毒 7 载量。
DOI：
10.1093/infdis/174.2.396
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Emery,VC
中科院分区：
文献类型：
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作者：
Kidd,IM;Clark,DA;Ait-Khaled,M;Griffiths,PD;Emery,VC
针对人类疱疹病毒 7 (HHV-7) 开发了定性和竞争定量巢式聚合酶链反应 (PCR) 检测方法。这些测定扩增了编码人类疱疹病毒 6 (HHV-6) U42 基因的 HHV-7 同源物部分的 DNA 序列。 PCR 检测用于分析 24 名健康志愿者的外周血 DNA (pbDNA) 和唾液。唾液中HHV-7的流行率为96%，中位病毒载量为1.1×106copies/mL。纵向分析显示病毒载量持续存在，表明病毒复制持续活跃。对 1 µg pbDNA 的分析显示，HHV-7 的患病率为 83%，病毒载量中位数为 40 个拷贝（267 个拷贝/106 个细胞）。对连续 pbDNA 样本的分析表明，个体的 HHV-7 病毒载量水平稳定。这些数据证明了 HHV-7 在两个不同位点的持久性，并提供了基线数据，可以与免疫功能低下患者的 HHV-7 负载进行比较。
Qualitative and competitive-quantitative nested polymerase chain reaction (PCR) assays were developed for human herpesvirus 7 (HHV-7). These assays amplify a DNA sequence encoding part of the HHV-7 homologue of the human herpesvirus 6 (HHV-6) U42 gene. The PCR assays were used to analyze peripheral blood DNA (pbDNA) and saliva from 24 healthy volunteers. The prevalence of HHV-7 in saliva was 96%, with a median virus load of 1.1 × 106copies/mL. Longitudinal analysis revealed sustained virus load, suggesting continued active viral replication. Analysis of 1 µg of pbDNA showed the prevalence of HHV-7 to be 83%, with a median virus load of 40 copies (267 copies/106cells). Analysis of sequential pbDNA samples showed individuals to have stable levels of HHV-7 virus load. These data demonstrate persistence of HHV-7 at two distinct sites and provide baseline data allowing comparisons with HHV-7 load in immunocompromised patients.