Increased number of CD25+ FoxP3+ regulatory T cells in oral squamous cell carcinomas detected by chromogenic immunohistochemical double staining
Increased number of CD25+ FoxP3+ regulatory T cells in oral squamous cell carcinomas detected by chromogenic immunohistochemical double staining
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DOI：
10.1111/j.1600-0714.2008.00641.x
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发表时间：
2008-09-01
影响因子：
3.3
通讯作者：
Driemel, Oliver
中科院分区：
文献类型：
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作者：
Schwarz, Stephan;Butz, Martin;Driemel, Oliver
背景：肿瘤浸润调节性T细胞（Treg）在口腔鳞状细胞癌（OSCC）中影响免疫细胞抗肿瘤作用的作用在很大程度上是未知的。目的：验证OSCC中CD25(+) FoxP3(+) Treg以及CD3(+) FoxP3（+）和CD8(+) FoxP3（+）肿瘤浸润淋巴细胞（TIL）的存在，并与非癌性淋巴上皮组织进行比较。结果：对15例OSCC组织切片进行CD3/FoxP3、CD8/FoxP3和CD25/FoxP3三组双染色，并与15例人扁桃体进行对比。结果：OSCC活检样本提供了TIL强浸润的证据，特别是自然发生的CD25(+) FoxP3(+) Treg。在OSCC和对照组织中，CD3(+) FoxP3(+) TIL和CD8(+) FoxP3(+) TIL的数量没有显著变化，但在OSCC中，CD25(+) FoxP3(+) TIL （Treg）的频率显著增加（P < 0.001，双侧t检验）。由于标本数量少，与肿瘤分期的相关性尚不能得到证实。结论：CD4/FoxP3双显色染色是检测鳞癌石蜡包埋组织Treg的一种很有前途的方法。
BACKGROUND: The role of tumor-infiltrating regulatory T cells (Treg) compromising antitumor effects of immune cells in oral squamous cell carcinoma (OSCC) is largely unknown.PURPOSE: The presence of CD25(+) FoxP3(+) Treg as well as of CD3(+) FoxP3(+) and of CD8(+) FoxP3(+) tumor-infiltrating lymphocytes (TIL) was verified in OSCC and compared with non-cancerous lymphoepithelial tissue.RESULTS: Three double stainings (CD3/FoxP3, CD8/FoxP3 and CD25/FoxP3) were performed on tissue sections of 15 OSCC and compared with 15 human tonsils.RESULTS: OSCC biopsy samples provide evidence for a strong infiltration of TIL, in particular, naturally occurring CD25(+) FoxP3(+) Treg. Whereas a comparison of OSCC and control tissue did not show significant changes in the number of CD3(+) FoxP3(+) TIL and of CD8(+) FoxP3(+) TIL, a significantly higher frequency of CD25(+) FoxP3(+) TIL (Treg) could be observed in OSCC (P < 0.001, two-sided t-test). Given the small number of specimens, a significant correlation with tumor stage could not be verified.CONCLUSION: Chromogenic double staining of CD4/FoxP3 is a promising tool for the detection of Treg in paraffin-embedded tissue of OSCC.