A species of small antisense RNA in posttranscriptional gene silencing in plants
A species of small antisense RNA in posttranscriptional gene silencing in plants
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DOI：
10.1126/science.286.5441.950
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发表时间：
1999-10-29
期刊：
影响因子：
56.9
通讯作者：
Baulcombe, DC
中科院分区：
文献类型：
--
作者：
Hamilton, AJ;Baulcombe, DC
转录后基因沉默（PTGS）是一种核苷酸序列特异性的防御机制，可以靶向细胞和病毒的mRNA。在此，分析了植物中三种类型的转基因诱导的PTGS和一个病毒诱导的PTGS的实例，在每种情况下，检测到与靶mRNA互补的反义RNA。这些RNA分子具有统一的长度，估计为25个核苷酸，并且它们的积累需要转基因正义转录或RNA病毒复制。因此，25个核苷酸的反义RNA可能是从RNA模板合成的，并且可能代表PTGS的特异性决定簇。
Posttranscriptional gene silencing (PTGS) is a nucleotide sequence-specific defense mechanism that can target both cellular and viral mRNAs. Here, three types of transgene-induced PTGS and one example of virus-induced PTGS were analyzed in plants, In each case, antisense RNA complementary to the targeted mRNA was detected. These RNA molecules were of a uniform Length, estimated at 25 nucleotides, and their accumulation required either transgene sense transcription or RNA virus replication. Thus, the 25-nucleotide antisense RNA is Likely synthesized from an RNA template and may represent the specificity determinant of PTGS.