An Electrochemical Compact Disk-type Microfluidics Platform for Use as an Enzymatic Biosensor
An Electrochemical Compact Disk-type Microfluidics Platform for Use as an Enzymatic Biosensor
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DOI：
10.1002/elan.201400590
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发表时间：
2015-03
期刊：
影响因子：
3
通讯作者：
P. Rattanarat;Prinjaporn Teengam;W. Siangproh;R. Ishimatsu;K. Nakano;O. Chailapakul;T. Imato
中科院分区：
文献类型：
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作者：
P. Rattanarat;Prinjaporn Teengam;W. Siangproh;R. Ishimatsu;K. Nakano;O. Chailapakul;T. Imato
描述了一种新型的基于离心的微流控装置与电化学检测器耦合，用于测定对照人血清中的葡萄糖。电化学光盘（eCD）平台是基于一个聚（二甲基硅氧烷）（PDMS）的材料，含有储存器，混合室，螺旋通道，碳糊电极（CPE）检测器，和废物储存器。对于电极制造，将由酞菁钴（CoPC）、石墨粉末、PDMS和矿物油组成的混合物印刷并配制成基于PDMS的电极图案。为了提高电化学灵敏度，将石墨烯-聚苯胺（G-PANI）纳米复合材料溶液浇铸到工作电极表面。在eCD平台以1000 rpm的转速旋转期间，在螺旋通道中混合分离的储器中的葡萄糖溶液和葡萄糖氧化酶溶液，以通过酶促反应产生过氧化氢。使用电极检测器测定产生的过氧化氢，所述电极检测器设定在相对于CPE的+0.4V的施加电位（伪参比电极）。在最佳条件下，线性校准范围为1至10 mM，检测限（LOD）为0.29 mM（S/N=3），定量限（LOQ）为0.97 mM（S/N=10）。检测了各种常见干扰化合物，包括抗坏血酸、尿酸、对乙酰氨基酚和L-半胱氨酸。最后，通过电流法测定并验证含有认证浓度的对照血清样品中的葡萄糖。使用eCD系统测量的葡萄糖水平与经认证的参考血清样本的实际值相匹配，准确度令人满意。
A novel centrifuge-based microfluidic device coupled with an electrochemical detector for the determination of glucose in control human serum is described. The electrochemical compact disk (eCD) platform was based on a poly(dimethylsiloxane) (PDMS) material containing reservoir, a mixing chamber, a spiral channel, a carbon-paste electrode (CPE) detector, and a waste reservoir. For electrode fabrication, a mixture consisting of cobalt phthalocyanine (CoPC), graphite powder, PDMS, and mineral oil was printed and formulated into a PDMS-based electrode pattern. To enhance electrochemical sensitivity, a graphene-polyaniline (G-PANI) nanocomposite solution was cast onto the working electrode surface. During the rotation of the eCD platform at a rotation speed of ∼1000 rpm, a glucose solution and a glucose oxidase solution in separated reservoirs were mixed in a spiral channel to produce hydrogen peroxide by an enzymatic reaction. The produced hydrogen peroxide was determined using the electrode detector set at an applied potential of +0.4 V vs. CPE (pseudo reference electrode). Under optimal conditions, a linear calibration ranging from 1 to 10 mM with a limit of detection (LOD) of 0.29 mM (S/N=3) and a limit of quantitation (LOQ) of 0.97 mM (S/N=10) was obtained. Various common interference compounds including ascorbic acid, uric acid, paracetamol, and L-cysteine were tested. Finally, glucose in control serum samples containing certified concentrations were amperometrically determined and validated. Glucose levels measured using the eCD system matched actual values for the certified reference serum samples with satisfactory accuracy.