Synaptic Vesicle Clusters at Synapses: A Distinct Liquid Phase?
Synaptic Vesicle Clusters at Synapses: A Distinct Liquid Phase?
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DOI：
10.1016/j.neuron.2017.02.013
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发表时间：
2017-03-08
期刊：
影响因子：
16.2
通讯作者：
De Camilli P
中科院分区：
文献类型：
--
作者：
Milovanovic D;De Camilli P
细胞质中的相分离正在成为细胞内组织的主要原理。在这个过程中，大分子集合自身组装成与周围介质不同但不受膜边界限制的液体隔室。在这里，我们讨论了相分离（其中两个相之一的成分是囊泡而不是大分子）如何成为突触前位点附近突触囊泡（SV）簇形成的基础。将SV组织成液相可以解释SV如何保持紧密聚集而不稳定地结合到支架上，使得它们可以被活性区组分有效地募集到释放位点。Milovanovic和De Camilli讨论了如何通过在细胞质内形成独特的液相来解释突触囊泡在突触处的聚集。这种组织结构可以解释突触囊泡是如何紧密地堆积在一起的，但在簇内是移动的。
Phase separation in the cytoplasm is emerging as a major principle in intracellular organization. In this process, sets of macromolecules assemble themselves into liquid compartments that are distinct from the surrounding medium but are not delimited by membrane boundaries. Here, we discuss how phase separation, where a component of one of the two phases is vesicles rather than macromolecules, could underlie the formation of synaptic vesicle (SV) clusters in proximity of presynaptic sites. The organization of SVs into a liquid phase could explain how SVs remain tightly clustered without being stably bound to a scaffold so that they can be efficiently recruited to release site by active zone components. Milovanovic and De Camilli discuss how clustering of synaptic vesicles at synapses could be accounted by their forming a distinct liquid phase within the cytoplasm. This organization may explain how synaptic vesicles are tightly packed, yet mobile, within clusters.