TRICHOME DEVELOPMENT IN ARABIDOPSIS-THALIANA .1. T-DNA TAGGING OF THE GLABROUS1 GENE
TRICHOME DEVELOPMENT IN ARABIDOPSIS-THALIANA .1. T-DNA TAGGING OF THE GLABROUS1 GENE
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DOI：
10.1105/tpc.1.11.1043
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发表时间：
1989-11-01
期刊：
影响因子：
11.6
通讯作者：
FELDMANN, KA
中科院分区：
文献类型：
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作者：
MARKS, MD;FELDMANN, KA
发现来自转化的拟南芥植物（通过农杆菌介导的种子转化程序产生）的后代分离为改变的毛状体表型。突变体植物具有正常的叶毛状体，但完全缺乏通常在茎上发现的毛状体。该突变与T-DNA插入物紧密相连。与遗传特征的毛状体突变体的互补分析表明，新的突变是GL 1位点的等位基因。新的毛状体突变体被命名为gl 1 -43。DNA凝胶印迹分析表明，插入位点包含一个复杂的阵列，至少有四个串联连接的T-DNA单元定向为直接和反向重复。使用来自gl 1 -43植物的DNA构建的基因组文库用于克隆T-DNA插入物左端侧翼的DNA。从插入中断的区域的DNA的可用性允许野生型GL 1基因的初步表征，并将允许最终克隆和测序这个发育有趣的基因。
Progeny from a transformed Arabidopsis plant (produced by the Agrobacterium-mediated seed transformation procedure) were found to be segregating for an altered trichome phenotype. The mutant plants have normal leaf trichomes but completely lack trichomes usually found on the stem. The mutation is tightly linked to a T-DNA insert. Complementation analysis with genetically characterized trichome mutants revealed that the new mutation is an allele of the GL1 locus. The new trichome mutant has been designated gl1-43. DNA gel blot analysis indicated that the insert site contains a complex array of at least four tandemly linked T-DNA units oriented as both direct and inverted repeats. A genomic library, constructed using DNA from gl1-43 plants, was used to clone DNA that flanks the left end of the T-DNA insert. The availability of DNA from the region interrupted by the insert has allowed initial characterization of the wild-type GL1 gene and will permit the eventual cloning and sequencing of this developmentally interesting gene.