IMMUNOHISTOCHEMICAL LOCALIZATION OF MINOR GANGLIOSIDES IN THE RAT CENTRAL-NERVOUS-SYSTEM
IMMUNOHISTOCHEMICAL LOCALIZATION OF MINOR GANGLIOSIDES IN THE RAT CENTRAL-NERVOUS-SYSTEM
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DOI：
10.1093/glycob/4.6.855
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发表时间：
1994-12-01
期刊：
影响因子：
4.3
通讯作者：
TAI, T
中科院分区：
文献类型：
--
作者：
KOTANI, M;KAWASHIMA, I;TAI, T
我们先前描述了通过特异性抗体检测的成年大鼠脑中主要神经节苷脂（GM 1、GD 1a、GD 1b、GT 1b和GQ 1b）的差异分布（Kotani，M.，川岛岛，小泽岛，Terashima，T. Tai，T. Glycobiology，3，137-146，1993）。在这里，我们报告的分布在成年大鼠大脑中的小神经节苷脂的免疫荧光技术与小鼠单克隆抗体（MAbs）。使用分别特异性识别GM 3、GM 2、GT 1a、GD 3、O-Ac-二唾液酸神经节苷脂、GD 2、GM 1b、GM 4、IV（3）NeuAc α-nLe（4）Cer和IV（6）NeuAc α-nLc（4）Cer的10种MAb（GMR 6、GMB 28、GMR 11、GMR 19、GMR 2、GMR 7、GGR 51、AMR 10、NGR 54和NGR 53）。我们的研究表明，有一个细胞类型特异性表达的次要神经节苷脂，以及主要的神经节苷脂，在大鼠脑。在小脑皮质，GM 3在白色物质中表达较强，在颗粒层中表达较弱。GD 3存在于颗粒层和白色物质中，但不在浦肯野细胞层或分子层中。一个O-Ac-disialoganglioside，这被认为是O-Ac-LD 1，被检测到专门在分子层和浦肯野细胞层。GD 2的存在仅限于颗粒层。GM 4与一些星形胶质细胞相关，但与髓鞘或少突胶质细胞无关。GM 2、GT 1a、GM 1b、IV（3）NeuAc alpha-nLc（4）Cer和IV（6）NeuAc alpha-nLc（4）Cer神经节苷脂在小脑皮质中的表达不明显。在其他区域，如大脑皮质、海马结构和脊髓，神经节苷脂的表达也高度定位于特定的细胞类型和层。
We previously described the differential distribution of major gangliosides (GM1, GD1a, GD1b, GT1b and GQ1b) in adult rat brain detected by specific antibodies (Kotani, M., Kawashima, I., Ozawa, I., Terashima, T. and Tai, T. Glycobiology, 3, 137-146, 1993). We report here the distribution of minor gangliosides in the adult rat brain by an immunofluorescence technique with mouse monoclonal antibodies (MAbs). Ten MAbs (GMR6, GMB28, GMR11, GMR19, GMR2, GMR7, GGR51, AMR10, NGR54 and NGR53) that specifically recognize GM3, GM2, GT1a, GD3, O-Ac-disialoganglioside, GD2, GM1b, GM4, IV(3)NeuAc alpha-nLe(4)Cer and IV(6)NeuAc alpha-nLc(4)Cer, respectively, were used. Our study revealed that there is a cell type-specific expression of minor gangliosides, as well as major gangliosides,in the rat brain. In the cerebellar cortex, GM3 was expressed intensely in the white matter and slightly in the granular layer. GD3 was present in both the granular layer and the white matter, but not in the Purkinje cell layer or in the molecular layer. An O-Ac-disialoganglioside, which was suggested to be O-Ac-LD1, was detected exclusively in both the molecular layer and Purkinje cell layer. The presence of GD2 was restricted to the granular layer. GM4 was associated with some astrocytes, but not with myelin or oligodendrocytes. GM2, GT1a, GM1b, IV(3)NeuAc alpha-nLc(4)Cer and IV(6)NeuAc alpha-nLc(4)Cer gangliosides were not clearly detected in the cerebellar cortex. In other regions, such as cerebral cortex, hippocampal formation and spinal cord, the expression of the gangliosides was also highly localized to a specific cell type and layer.