Differential effects of propionate or β-hydroxybutyrate on genes related to energy balance and insulin sensitivity in bovine white adipose tissue explants from a subcutaneous and a visceral depot.
Differential effects of propionate or β-hydroxybutyrate on genes related to energy balance and insulin sensitivity in bovine white adipose tissue explants from a subcutaneous and a visceral depot.
复制标题
丙酸盐或β-羟基丁酸盐对来自皮下和内脏库的牛白色脂肪组织外植体中与能量平衡和胰岛素敏感性相关的基因的不同影响。
DOI：
--
复制
发表时间：
2012
影响因子：
2.7
通讯作者：
M. Mielenz
中科院分区：
文献类型：
--
作者：
A. Hosseini;C. Behrendt;P. Regenhard;H. Sauerwein;M. Mielenz
反刍动物依靠短链脂肪酸（SCFA）作为主要能量来源。在此，我们比较了丙酸盐、β-羟基丁酸盐（BHB）和胰岛素对体外培养的牛皮下（SC）和腹膜后（RP）脂肪组织（AT）外植体中能量平衡相关基因mRNA丰度的影响。与对照相比，丙酸盐显著（p < 0.05）或作为趋势（p ≤ 0.1）影响处理样品中两个贮库中基因（如脂联素系统）的mRNA丰度。丙酸盐仅在SC AT中增加脂联素受体1（AdipoR 1）和AdipoR 2 mRNA。β-羟基丁酸有降低SC AT中脂联素和AdipoR 1 mRNA丰度的趋势。游离脂肪酸受体2/3（FFAR 2/3）和其他相关基因（GOI）的mRNA丰度在牛前脂肪细胞培养的分化过程中增加。短期胰岛素刺激后，所有GOI的mRNA丰度保持不变。总之，丙酸盐、BHB或胰岛素在短期治疗期间对两个储库中GOI的mRNA丰度产生不同的影响。我们的研究结果表明，牛脂联素系统可能是更敏感的丙酸比BHB。我们证明了FFAR 2/3 mRNA的存在不仅在两个AT仓库，但也在分化前脂肪细胞分离牛SC AT。因此，我们确定SCFA能够对牛脂肪组织产生胰岛素非依赖性作用，这可能独立于丙酸盐摄取相关事件。
Ruminants rely on short-chain fatty acids (SCFA) as principal energy source. Herein, we compared the effects of propionate, β-hydroxybutyrate (BHB) and insulin on mRNA abundance of energy balance-related genes by short-term incubation (4 h) in bovine subcutaneous (SC) and retroperitoneal (RP) adipose tissue (AT) explants in vitro. Propionate either significantly (p < 0.05), or as a trend (p ≤ 0.1) affected mRNA abundance of genes such as adiponectin system in both depots in treated samples versus controls. Propionate increased adiponectin receptor 1 (AdipoR1) and AdipoR2 mRNA only in SC AT. β-hydroxybutyrate decreased mRNA abundance of adiponectin and AdipoR1 in SC AT as a trend. The mRNA abundance of free fatty acid receptor 2/3 (FFAR2/3) and other genes of interest (GOI) was increased during differentiation in bovine preadipocyte culture. The mRNA abundance of all the GOI remained unchanged after short-term insulin stimulation. In total, propionate, BHB or insulin during short-term treatment exert divergent effects on the mRNA abundance of GOI in both depots in vitro. Our results indicate that the bovine adiponectin system might be more sensitive to propionate than to BHB. We demonstrated the presence of FFAR2/3 mRNA not only in both AT depots but also in differentiating preadipocytes isolated from bovine SC AT. Therefore, we established that SCFA are able to exert insulin-independent effects on bovine adipose tissue, which might be independent from propionate uptake-related events.
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1073/pnas.95.11.5987
发表时间：
1998-05-26
影响因子：
11.1
作者：
Horton, JD;Bashmakov, Y;Shimano, H
通讯作者：
Shimano, H
影响因子：
11.2
作者：
Thangaraju M;Cresci GA;Liu K;Ananth S;Gnanaprakasam JP;Browning DD;Mellinger JD;Smith SB;Digby GJ;Lambert NA;Prasad PD;Ganapathy V
通讯作者：
Ganapathy V