Development of a novel biomarker of free radical damage in reperfusion injury after cardiac arrest
Development of a novel biomarker of free radical damage in reperfusion injury after cardiac arrest
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DOI：
10.1016/s0014-5793(00)01279-5
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发表时间：
2000-03-17
期刊：
影响因子：
3.5
通讯作者：
Wiklund, L
中科院分区：
文献类型：
--
作者：
Basu, S;Nozari, A;Wiklund, L
在猪心肺复苏(CPR)模型中，我们研究了12头仔猪在心脏骤停后复苏期间血浆氧化损伤标志物8-iso-PGF(2α)和炎症反应指标15-酮二氢PGF(2α)水平的变化。6只未发生心脏骤停的仔猪作为对照。在VF5组，颈静脉球部血浆(引流脑)中8-iso-PGF(2α)增加了4倍。对照组颈静脉球部8-iso-PGF(2α)无明显变化。VF5组的15-酮-二氢前列腺素F(2α)也增加了4倍。因此，颈静脉球部血浆中8-iso-PGF(2α)和15-酮-二氢PGF(2α)的测定可作为定量研究自由基氧化脑损伤和再灌注损伤炎症反应的生物标志物。(C)2000年欧洲生化学会联合会。
In a porcine model of cardiopulmonary resuscitation (CPR), we investigated changes in the plasma levels of 8-iso-PGF(2 alpha), a marker for oxidative injury, and 15-keto-dihydro-PGF(2 alpha), an inflammatory response indicator during the postresuscitation period after cardiac arrest, Twelve piglets were subjected to either 2 or 5 min (VF2 and VF5 group) of ventricular fibrillation (VF) followed by 5 min of closed-chest CPR. Six piglets without cardiac arrest were used as controls. In VF5 group, 8-iso-PGF(2 alpha), in the jugular bulb plasma (draining the brain) increased four-fold. Jugular bulb 8-iso-PGF(2 alpha), in the control group remained unchanged. The 15-keto-dihydro-PGF(2 alpha), also increased four-fold in the VF5 group. Thus, 8-iso-PGF(2 alpha), and 15-keto-dihydro-PGF(2 alpha), measurements in jugular bulb plasma may be used as biomarkers for quantification of free radical catalyzed oxidative brain injury and inflammatory response in reperfusion injury. (C) 2000 Federation of European Biochemical Societies.