Expression analysis of the mixed function oxidase system in rat brain by the polymerase chain reaction.
Expression analysis of the mixed function oxidase system in rat brain by the polymerase chain reaction.
复制标题
通过聚合酶链式反应分析大鼠脑中混合功能氧化酶系统的表达。
DOI：
10.1007/bf00926090
复制
发表时间：
1993
影响因子：
4.3
通讯作者：
Strobel,HW
中科院分区：
文献类型：
--
作者：
Hodgson,AV;White,TB;White,JW;Strobel,HW
治疗药物在体内通过混合功能氧化酶系统的代谢是分析药物有效性的重要考虑因素。神经特异性药物在脑内的 P450 依赖性代谢可能会影响药物的作用过程。为了确定细胞色素 P450 是否在大脑中表达，从用多种已知的肝脏 P450 诱导剂（包括阿米替林、丙咪嗪、异黄樟素、苯巴比妥和 β-萘黄酮）治疗的大鼠的整个大脑中分离出 RNA。通过 PCR 技术分析 RNA 中 P450 同工酶的存在。根据治疗的不同，在大脑样本中检测到 P450IA1、P450IIB1、P450IIB2、P450IID 和 P450IIE1 的差异表达。在大脑样本中也检测到了细胞色素 P450 还原酶的表达，这有力地证明了大脑在所有研究条件下都含有一个有效的混合功能氧化酶系统。 （摩尔细胞生物化学 120：171–179，1993）
Metabolism of therapeutic drugs in the body by the mixed function oxidase system is an important consideration in the analysis of a drug's effectiveness. P450-dependent metabolism within the brain of a neuro-specific drug may affect the drug's course of action. To determine whether cytochrome P450 was expressed in brain, RNA was isolated from the whole brains of rats treated with a variety of known hepatic P450 inducers, including amitriptyline, imipramine, isosafrole, phenobarbital, and β-naphthoflavone. The RNA was analyzed for the presence of P450 isozymes by the PCR technique. Differential expression of P450IA1, P450IIB1, P450IIB2, P450IID, and P450IIE1 was detected in the brain samples, depending on the treatment. Cytochrome P450 reductase expression was also detected in the brain samples, giving strong evidence that the brain contains a competent mixed function oxidase system under all conditions studied. (Mol Cell Biochem120: 171–179, 1993)