EPSTEIN-BARR VIRUS RECEPTOR OF HUMAN LYMPHOCYTES-B IS THE C3D RECEPTOR CR-2
EPSTEIN-BARR VIRUS RECEPTOR OF HUMAN LYMPHOCYTES-B IS THE C3D RECEPTOR CR-2
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DOI：
10.1073/pnas.81.14.4510
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
FEARON, DT
中科院分区：
文献类型：
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作者：
FINGEROTH, JD;WEIS, JJ;FEARON, DT
使用单克隆抗体 HB-5 和抗 B2 进行 3 组实验，确定了 Epstein-Barr 病毒 (EBV) 受体与 2 型补体受体 (CR2) 的同一性，这些抗体识别分子量为 145,000 的 B 淋巴细胞膜蛋白 CR2。首先，荧光素化 EBV 与 4 个类淋巴母细胞系（SB、JY、Raji 和 Molt-4）结合的排序与分析流式细胞术测定的 HB-5 和抗 B2 结合的排序相同。其次，用 HB-5 预处理细胞，然后用针对小鼠 IgG 的山羊 F(ab'')2 片段处理，可阻断荧光素化 EBV 在 SB（一种 B 类淋巴母细胞系）上的结合。单独的 HB-5、单独的二抗、兔抗 C3b 受体或 UPC10（一种不相关的单克隆抗体）不会抑制病毒附着。第三，将CR2从SB转移到已吸收HB-5的携带蛋白A的金黄色葡萄球菌颗粒上，赋予它们结合125I标记的EBV的特异性能力。显然，CR2是人B淋巴细胞的EBV受体。
Identity of the Epstein-Barr virus (EBV) receptor with the complement receptor type 2 (CR2) was established in 3 sets of experiments using the monoclonal antibodies, HB-5 and anti-B2, which recognize a MW 145,000 B-lymphocyte membrane protein that is CR2. First, the rank order for binding of fluoresceinated EBV to 4 lymphoblastoid cell lines (SB, JY, Raji and Molt-4) was identical to the rank order for binding of HB-5 and anti-B2 by analytical flow cytometry. Second, pretreatment of cells with HB-5 followed by treatment with goat F(ab'')2 fragments to mouse IgG blocked binding of fluoresceinated EBV on SB, a B-lymphoblastoid cell line. Virus attachment was not inhibited by HB-5 alone, second antibody alone, rabbit anti-C3b receptor or UPC10 (an irrelevant monoclonal antibody). Third, transfer of CR2 from SB to protein A-bearing Staphylococcus aureus particles, to which HB-5 had been absorbed, conferred on them the specific ability to bind 125I-labeled EBV. Evidently, CR2 is the EBV receptor of human B lymphocytes.