G3BP1 may serve as a potential biomarker of proliferation, apoptosis, and prognosis in oral squamous cell carcinoma
G3BP1 may serve as a potential biomarker of proliferation, apoptosis, and prognosis in oral squamous cell carcinoma
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G3BP1可能作为口腔鳞状细胞癌增殖、凋亡和预后的潜在生物标志物
DOI：
10.1111/jop.13199
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发表时间：
2021-05-27
影响因子：
3.3
通讯作者：
Su, Tong
中科院分区：
文献类型：
--
作者：
Hu, Xin;Xia, Kun;Su, Tong
背景G3 BP 1是许多类型癌症的预后生物标志物;然而，其在口腔鳞状细胞癌中的作用仍不清楚。方法采用免疫组化方法检测G3 BP 1、E-cadherin、Ki 67、Ki 67、Ki 6和Cleaved-Caspase 3。结果G3 BP 1蛋白在口腔鳞癌组织中表达显著上调，与Ki 67呈正相关，与Cleaved-Caspase 3呈负相关。基于在线数据库中的信息，G3 BP 1 mRNA水平在口腔鳞状细胞癌中显著高于正常组织。高G3 BP 1 mRNA水平与口腔鳞状细胞癌患者的总体生存率相关。富集分析表明G3 BP 1参与解旋酶/催化/ATP酶活性功能和剪接体/RNA转运/细胞周期途径。结论G3 BP 1可作为口腔鳞癌细胞增殖、凋亡及预后的生物学指标。
Background G3BP1 is a prognostic biomarker for many types of cancers; however, its role in oral squamous cell carcinoma remains unclear. We investigated the role of G3BP1 as a potential biomarker for proliferation, apoptosis, and prognosis in oral squamous cell carcinoma.Methods We obtained samples of normal oral mucosa (n = 17), oral squamous cell carcinoma tissues (n = 61), and paired adjacent tissues (n = 47) from Xiangya Hospital for immunohistochemical evaluation to measure the expression of G3BP1, E-cadherin, Ki67, and Cleaved-caspase3. Using data from The Cancer Genome Atlas, we performed bioinformatics analysis to investigate the prognosis, functions, signaling pathways, and immune infiltrate significance related to G3BP1 in oral squamous cell carcinoma.Results The G3BP1 protein level was significantly upregulated in oral squamous cell carcinoma tissues and was also positively associated with Ki67 and negatively associated with Cleaved-caspase3. Based on information available in online database, the G3BP1 mRNA level was significantly higher in oral squamous cell carcinoma than in normal tissues. High G3BP1 mRNA levels were associated with poor overall survival rates in patients with oral squamous cell carcinoma. Enrichment analysis showed that G3BP1 was involved in the helicase/catalytic/ATPase activity functions and spliceosome/RNA transport/ cell cycle pathways. Furthermore, G3BP1 mRNA levels were positively associated with CD4(+) T-cell infiltration.Conclusions G3BP1 may serve as a potential biomarker for proliferation, apoptosis, and prognosis of oral squamous cell carcinoma.