A dermal niche for multipotent adult skin-derived precursor cells
A dermal niche for multipotent adult skin-derived precursor cells
复制标题
DOI：
10.1038/ncb1181
复制
发表时间：
2004-11-01
影响因子：
21.3
通讯作者：
Miller, FD
中科院分区：
文献类型：
--
作者：
Fernandes, KJL;McKenzie, IA;Miller, FD
干细胞研究中的一个基本问题是培养的多能成体干细胞是否代表内源性多能前体细胞。在这里，我们解决这个问题，专注于SKPs，培养的成人干细胞从真皮产生神经和中胚层后代。我们表明，SKPs来自内源性成人真皮前体细胞，表现出类似于胚胎神经嵴干细胞的特性。我们证明，这些内源性SKPs可以首先从胚胎发育过程中的皮肤中分离出来，并持续到成年期，在毛须毛囊的乳头中有一个小生境。此外，谱系分析表明，毛发和胡须毛囊真皮乳头含有神经嵴来源的细胞，从胡须垫SKPs的神经嵴起源。我们认为SKPs代表了一种内源性胚胎前体细胞，在发育过程中出现在外周组织如皮肤中，并保持多能性直至成年。
A fundamental question in stem cell research is whether cultured multipotent adult stem cells represent endogenous multipotent precursor cells. Here we address this question, focusing on SKPs, a cultured adult stem cell from the dermis that generates both neural and mesodermal progeny. We show that SKPs derive from endogenous adult dermal precursors that exhibit properties similar to embryonic neural-crest stem cells. We demonstrate that these endogenous SKPs can first be isolated from skin during embryogenesis and that they persist into adulthood, with a niche in the papillae of hair and whisker follicles. Furthermore, lineage analysis indicates that both hair and whisker follicle dermal papillae contain neural-crest-derived cells, and that SKPs from the whisker pad are of neural-crest origin. We propose that SKPs represent an endogenous embryonic precursor cell that arises in peripheral tissues such as skin during development and maintains multipotency into adulthood.