Dietary Cholesterol Atherogenic Changes in Juvenile Rabbits
Dietary Cholesterol Atherogenic Changes in Juvenile Rabbits
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DOI：
10.1248/bpb.b14-00775
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发表时间：
2015-05-01
影响因子：
2
通讯作者：
Yoshikawa, Tomohiro
中科院分区：
文献类型：
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作者：
Keyamura, Yuka;Nagano, Chifumi;Yoshikawa, Tomohiro
动脉粥样硬化病变的形成始于胎儿的发育，并在青春期后随着年龄的增长而发展。然而，对幼年期动脉粥样硬化形成的研究还不够深入。在这项研究中，我们检测了幼兔对胆固醇喂养的动脉粥样硬化易感性。8周龄(青年组)和12周龄(老年组)雄性新西兰大白兔分别饲喂含胆固醇0.5%的饲料8周，观察动脉粥样硬化斑块面积。血浆总胆固醇、甘油三酯和磷脂浓度在两组之间没有差异；然而，年轻组的高密度脂蛋白胆固醇浓度比老年组低23%。动脉粥样硬化病变面积在年轻组显著增大(32+/-21%)。然而，在老年组中，仅观察到这些区域的适度变化(3.3+/-0.3%)。组织学检查显示青年组兔主动脉病变内膜明显增厚，巨噬细胞聚集。据我们所知，这是第一次研究表明饮食中胆固醇诱导的动脉粥样硬化改变在幼兔身上发生的时间很短。
Atherosclerotic lesion formation starts during fetal development and progresses with age after adolescence. However, atherogenesis during the juvenile period has not been studied thoroughly. In this study, we examined the atherogenic susceptibility of juvenile rabbits to cholesterol feeding. Male New Zealand White rabbits aged 8 (younger group) and 12 (older group) weeks were fed a 0.5% cholesterol-containing diet for 8 weeks, and then their aortic atherosclerotic lesion areas were evaluated. Plasma concentrations of total cholesterol, triglycerides, and phospholipids did not differ between the two groups; however, plasma concentrations of high-density lipoprotein cholesterol were 23% lower in the younger than in the older group. Atherosclerotic lesion areas were significantly larger in the younger group (32 +/- 21%). However, only moderate changes were observed in these areas in the older group (3.3 +/- 0.3%). Histological examination showed marked intimal thickening and macrophage accumulation in the aortic lesions of rabbits in the younger group. To the best of our knowledge, this is the first study to show that dietary cholesterol-induced atherogenic changes markedly occur during a short period in juvenile rabbits.