Characterization of 47 Cys2-His2 zinc finger proteins required for the development and pathogenicity of the rice blast fungus Magnaporthe oryzae
Characterization of 47 Cys2-His2 zinc finger proteins required for the development and pathogenicity of the rice blast fungus Magnaporthe oryzae
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稻瘟病菌 Magnaporthe oryzae 发育和致病性所需的 47 个 Cys2 -His2 锌指蛋白的表征。
DOI：
10.1111/nph.13948
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发表时间：
2016-08-01
期刊：
影响因子：
9.4
通讯作者：
Lu, Jianping
中科院分区：
文献类型：
--
作者：
Cao, Huijuan;Huang, Pengyun;Lu, Jianping
Cys 2-His 2（C2 H2）锌指蛋白家族是稻瘟病菌中第二大的转录因子家族。然而，关于大多数C2 H2 TF在M. tumor的发育和致病性中的作用知之甚少。通过基因缺失的方法研究了47个C2 H2基因在微囊藻发育和致病中的作用。利用RNA测序和定量PCR（qPCR）分析菌丝体和附着胞中TF依赖基因。其中44个C2 H2基因与黑曲霉的生长（20个基因）、产孢（28个基因）、附着胞形成（4个基因）和致病性（22个基因）有关。其中，MGG_14931，命名为VRF 1，是致病性所必需的，通过影响与附着胞结构和功能相关的基因的表达，特异性地控制附着胞成熟，包括黑色素生物合成、几丁质催化、脂质代谢、蛋白水解、跨膜运输和对氧化胁迫的响应; MGG_01776，命名为VRF 2，是植物侵入和侵入生长所必需的;分生孢子相关基因CON 7是分生孢子分化所必需的; MoCREA编码碳分解代谢产物阻遏蛋白，是一种新的脂代谢阻遏物，当葡萄糖可在M.本研究为深入了解C2 H2转座子对烟草赤星病菌生长、无性发育、附着胞形成和致病性的调控提供了新的思路。
The Cys2 -His2 (C2H2) zinc finger protein family is the second-largest family of transcription factors (TFs) in Magnaporthe oryzae, the causal fungus responsible for the destructive rice blast disease. However, little is known about the roles of most C2H2 TFs in the development and pathogenicity of M. oryzae. The roles of 47 C2H2 genes in development and pathogenicity were investigated by gene deletion in M. oryzae. The TF-dependent genes in mycelia or appressoria were analyzed with RNA sequencing and quantitative PCR (qPCR). Forty-four C2H2 genes are involved in growth (20 genes), conidiation (28 genes), appressorium formation (four genes) and pathogenicity (22 genes) in M. oryzae. Of these, MGG_14931, named as VRF1, is required for pathogenicity, specifically controlling appressorium maturation by affecting the expression of genes related to appressorial structure and function, including melanin biosynthesis, chitin catabolism, lipid metabolism, proteolysis, transmembrane transport, and response to oxidative stress; MGG_01776, named as VRF2, is required for plant penetration and invasive growth; conidiation-related gene CON7 is required for conidial differentiation; and MoCREA, encoding a carbon catabolite repression protein, is a novel repressor of lipid catabolism when glucose obtainable in M. oryzae. This study provides many insights into the regulation of growth, asexual development, appressorium formation, and pathogenicity by C2H2 TFs in M. oryzae.