Long non-coding RNA ZFAS1 is an unfavourable prognostic factor and promotes glioma cell progression by activation of the Notch signaling pathway
Long non-coding RNA ZFAS1 is an unfavourable prognostic factor and promotes glioma cell progression by activation of the Notch signaling pathway
复制标题
DOI：
10.1016/j.biopha.2017.01.014
复制
发表时间：
2017-03-01
影响因子：
7.5
通讯作者：
Shao, Lianbin
中科院分区：
文献类型：
--
作者：
Gao, Kai;Ji, Zhiwu;Shao, Lianbin
胶质瘤患者的生存率仍然很低，这在很大程度上归因于活跃的癌变。越来越多的证据表明，长链非编码RNA（lncRNA）在肿瘤的发生和发展中起着关键作用。然而，lncRNA ZFAS 1在胶质瘤中的功能尚不清楚。在目前的研究中，我们发现ZFAS 1在胶质瘤组织和细胞系中上调。ZFAS 1在胶质瘤组织中的高表达与肿瘤的晚期和较差的总生存率显著相关。此外，体外试验表明，ZFAS 1抑制显着抑制胶质瘤细胞的增殖，迁移和侵袭。重要的是，我们进一步证实了上皮间质转化（EMT）和Notch信号通路在ZFAS 1敲低后在胶质瘤细胞中失活。因此，我们的研究结果表明，ZFAS 1可以通过调节EMT和Notch信号通路在胶质瘤的发展中发挥肿瘤致癌作用。LncRNA ZFAS 1有可能成为胶质瘤治疗的新靶点。(C)2017 Elsevier Masson SAS。All rights reserved.
Survival of patients with glioma remains poor, which is largely attributed to active carcinogenesis. Accumulating evidence indicates that long non-coding RNAs (lncRNAs) play key roles in tumor initiation and progression. However, the function of lncRNA ZFAS1 in glioma is still unclear. In the current study, we found that ZFAS1 was upregulated in glioma tissues and cell lines. High ZFAS1 expression in glioma tissues was significantly correlated with advanced tumor stage and poor overall survival. Furthermore, in vitro assays demonstrated that ZFAS1 inhibition significantly suppressed glioma cell proliferation, migration and invasion. Importantly, we further confirmed that epithelial-mesenchymal transition (EMT) and the Notch signaling pathway was inactivated in the glioma cells after ZFAS1 knockdown. Thus, our findings indicated that ZFAS1 could exhibit a tumor oncogenic role in glioma progression by regulating EMT and Notch signaling pathway. LncRNA ZFAS1 might serve as a therapeutic target for the treatment of glioma patients. (C) 2017 Elsevier Masson SAS. All rights reserved.