Anatomic Changes in Schlemm's Canal and Collector Channels in Normal and Primary Open-Angle Glaucoma Eyes Using Low and High Perfusion Pressures
Anatomic Changes in Schlemm's Canal and Collector Channels in Normal and Primary Open-Angle Glaucoma Eyes Using Low and High Perfusion Pressures
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DOI：
10.1167/iovs.14-14128
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发表时间：
2014-09-01
影响因子：
4.4
通讯作者：
Fautsch, Michael P.
中科院分区：
文献类型：
--
作者：
Hann, Cheryl R.;Vercnocke, Andrew J.;Fautsch, Michael P.
目的。检查低灌注压和高灌注压下正常人和原发性开角型青光眼 (POAG) 眼的施累姆氏管 (SC) 和收集通道 (CC) 的解剖结构。方法。在正常 (n = 3) 和 POAG (n = 3) 眼对中，一只眼以 10 mm Hg 灌注，而另一只眼以 20 mm Hg 灌注 2 小时。将眼睛灌注固定在相同的压力下，解剖成象限，嵌入 Epon Araldite 中，并通过三维微型计算机断层扫描 (3D micro-CT) 进行扫描。使用ANALYZE软件测量施累姆氏管体积、CC孔口面积、直径和数量。结果。与 20 mm Hg 相比，10 mm Hg 时正常眼显示出更大的 SC 体积（3.3 倍）和 CC 孔口面积（9962.8 vs. 8825.2 μm(2)）和相似的 CC 直径（34.3 +/- 17.8 vs. 32.7 +/- 13.0 μm）。在 POAG 眼中，与 20 mm Hg 灌注压相比，10 mm Hg 灌注压下 SC 体积（2.0 倍）、CC 孔口面积（8049.2 μ m(2) - 6468.4 μ m(2)）和 CC 直径（36.2 +/- 19.1 vs. 29.0 +/- 13.8 μm）增加。正常眼和 POAG 眼均存在部分和全部 CC 闭塞，在 20 mm Hg 条件下，与正常眼相比，POAG 眼的总闭塞增加了 3.7 倍。与 10 mm Hg 相比，20 mm Hg 下正常眼睛的 CC 可视化增加了 24%，POAG 眼睛的 CC 可视化增加了 21%。在相同压力下，与正常眼相比，POAG 眼的施莱姆管体积、CC 面积和 CC 直径均减小。结论。 POAG 眼中短暂和短期压力升高的补偿机制似乎减弱。 SC 和 CC 对压力变化的可变响应可能是 POAG 眼流出设施变化的一个影响因素。
PURPOSE. To examine the anatomy of Schlemm's canal (SC) and collector channels (CCs) in normal human and primary open-angle glaucoma (POAG) eyes under low and high perfusion pressure.METHODS. In normal (n = 3) and POAG (n = 3) eye pairs, one eye was perfused at 10 mm Hg while the fellow eye was perfused at 20 mm Hg for 2 hours. Eyes were perfusion fixed at like pressures, dissected into quadrants, embedded in Epon Araldite, and scanned by three-dimensional micro-computed tomography (3D micro-CT). Schlemm's canal volume, CC orifice area, diameter, and number were measured using ANALYZE software.RESULTS. Normal eyes showed a larger SC volume (3.3-fold) and CC orifice area (9962.8 vs. 8825.2 mu m(2)) and a similar CC diameter (34.3 +/- 17.8 vs. 32.7 +/- 13.0 mu m) at 10 mm Hg compared to 20 mm Hg. In POAG eyes, SC volume (2.0-fold), CC orifice area (8049.2 mu m(2) - 6468.4 mu m(2)), and CC diameter (36.2 +/- 19.1 vs. 29.0 +/- 13.8 mu m) were increased in 10 mm Hg compared to 20 mm Hg perfusion pressures. Partial and total CC occlusions were present in normal and POAG eyes, with a 3.7-fold increase in total occlusions in POAG eyes compared to normal eyes at 20 mm Hg. Visualization of CCs increased by 24% in normal and by 21% in POAG eyes at 20 mm Hg compared to 10 mm Hg. Schlemm's canal volume, CC area, and CC diameter were decreased in POAG eyes compared to normal eyes at like pressures.CONCLUSIONS. Compensatory mechanisms for transient and short periods of increased pressure appear to be diminished in POAG eyes. Variable response to pressure change in SC and CCs may be a contributing factor to outflow facility change in POAG eyes.