Effects of temperature on the size and shape of Escherichia coli cells
Effects of temperature on the size and shape of Escherichia coli cells
复制标题
温度对大肠杆菌细胞大小和形状的影响
DOI：
10.1007/bf00405885
复制
发表时间：
1982
影响因子：
2.8
通讯作者：
C. Woldringh
中科院分区：
文献类型：
--
作者：
F. J. Trueba;E. V. Spronsen;J. Traas;C. Woldringh
两种大肠杆菌亚株在不同的生长培养基中在 22 至 42°C 的温度下分批培养至稳态。细胞的大小和形状是通过光学和电子显微照片以及库尔特通道器测量的。结果表明，在较低温度下，细胞较短且稍厚，尤其是在丰富的生长培养基中；细胞体积则稍小。进一步发现较低的温度可以增加收缩期的相对持续时间。细胞尺寸分布的形状无法区分，表明细胞的生长模式在所有温度下都是相同的。细胞对温度变化的适应持续了几代，表明温度的形态效应是由细胞的生理学介导的。
Two substrains ofEscherichia coliB/r were grown to steady-state in batch cultures at temperatures between 22 and 42° C in different growth media. The size and shape of the cells were measured from light and electron micrographs and with the Coulter channelizer. The results indicate that cells are shorter and somewhat thicker at the lower temperatures, especially in rich growth media; cell volume is then slightly smaller. A lower temperature was further found to increase the relative duration of the constriction period. The shapes of the cell size distributions are indistinguishable, indicating that the pattern of growth of the cells is the same at all temperatures. The adaptation of the cells to a temperature shift lasted several generations, indicating that the morphological effects of temperature are mediated by the cell's physiology.