IMMUNOLOGICAL ASPECTS OF HUMAN COLOSTRUM AND MILK .2. CHARACTERISTICS OF LYMPHOCYTE-REACTIVITY AND DISTRIBUTION OF E-ROSETTE FORMING CELLS AT DIFFERENT TIMES AFTER ONSET OF LACTATION
IMMUNOLOGICAL ASPECTS OF HUMAN COLOSTRUM AND MILK .2. CHARACTERISTICS OF LYMPHOCYTE-REACTIVITY AND DISTRIBUTION OF E-ROSETTE FORMING CELLS AT DIFFERENT TIMES AFTER ONSET OF LACTATION
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DOI：
10.1016/s0022-3476(78)80286-8
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发表时间：
1978-01-01
影响因子：
5.1
通讯作者：
OGRA, PL
中科院分区：
文献类型：
--
作者：
OGRA, SS;OGRA, PL
在泌乳发作后的不同时间，研究了T [胸腺衍生]淋巴细胞和其他细胞的分布，以及其他细胞和其他细菌和病毒抗原的增殖活性。 E [红细胞] - 渗透液形成和体外淋巴增生性反应的技术用于研究初乳和牛奶和外周血淋巴细胞的反应。在产后第1天，在初乳中观察到总细胞（105-107/mL）和电子缓解淋巴细胞（104-105/mL）的数量最高。随后，细胞成分的浓度急剧降低，到产后第四天，牛奶中的电子棒形成细胞的数量下降了约10倍。产后180天的E-Rosette形成细胞的数量约为103/ml。在外周血中的细胞计数中未观察到变化。在初乳淋巴细胞中经常检测到对植物凝集素，康甘那藻素A，链霉菌酶，念珠菌，红宝石，巨细胞病毒和腮腺炎病毒抗原的增生反应。初乳细胞的活性似乎与外周血淋巴细胞的活性无关。尽管有足够数量的E-Rosette形成的细胞，但在产后15-180天收集的牛奶中几乎没有反应。初乳和牛奶对外周血以及初乳和牛奶淋巴细胞的增殖反应具有深远的抑制作用。
The distribution of T[thymus-derived]-lymphocytes and other cells and proliferative activity to a variety of bacterial and viral antigens was studied in the colostrum and milk and peripheral blood of postpartum subjects at various times after the onset of lactation. The techniques of E[erythrocyte]-rosette formation and in vitro lymphoproliferative responses were employed to study the responses in colostrum and milk and peripheral blood lymphocytes. The highest number of total cells (105-107/ml) and of E-rosetting lymphocytes (104-105/ml) were observed in the colostrum on the 1st postpartum day. Subsequently, the concentration of cellular components decreased sharply and by the 4th postpartum day the number of E-rosette forming cells in milk had dropped by about 10-fold; the number of E-rosette forming cells 180 days postpartum was about 103/ml. No change was observed in the cell counts in the peripheral blood. The proliferative responses to phytohemagglutinin, concanavalin A, streptokinase, Candida, rubella, cytomegalovirus and mumps virus antigens were frequently detected in the colostrum lymphocytes. The activity in colostrum lymphocytes appeared to be independent of the activity in the peripheral blood lymphocytes. Little or no responses were observed in the milk collected 15-180 days postpartum, in spite of an adequate number of E-rosette forming cells. Colostrum and milk had a profound inhibitory effect on the proliferative responses of peripheral blood and on colostrum and milk lymphocytes.