Solving an enigma: Arterial pole development in the zebrafish heart
Solving an enigma: Arterial pole development in the zebrafish heart
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DOI：
10.1016/j.ydbio.2005.11.042
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发表时间：
2006-02-15
影响因子：
2.7
通讯作者：
Kirby, ML
中科院分区：
文献类型：
--
作者：
Grimes, AC;Stadt, HA;Kirby, ML
人们普遍认为，斑马鱼心脏的动脉极在比较心脏发生的模型中是不寻常的。这部分是基于动脉球在发育过程中经历横纹肌到平滑肌表型转变的报道。这意味着，斑马鱼，一个几乎普遍接受的模型在其他领域的比较生物学，可能不适合研究锥干异常的人类疾病。然而，虽然房室特异性分子标记的使用已经允许广泛表征心房和心室的发育，但缺乏任何球特异性标记意味着斑马鱼心脏的该区域的特征很差，并且很可能被误解。我们已经发现，荧光一氧化氮指示剂4，5-二氨基荧光素二乙酸酯（<$A-2DA）特异性标记动脉球整个发展约48它受精后。因此，我们试图用β-2DA和免疫组织化学方法进一步描述球的发育。我们得出的结论是，没有发生这样的表型转变，与目前的想法相反，斑马鱼动脉极发育的各个方面在进化上是保守的，球不应该被认为是一个腔室，更类似于高等脊椎动物的动脉干。(C)2005年爱思唯尔公司All rights reserved.
It is a widely held belief that the arterial pole of the zebrafish heart is unusual among models of comparative cardiogenesis. This is based, in part, on the report that the bulbus arteriosus undergoes a striated-to-smooth muscle phenotypic transition during development. An implication of this is that the zebrafish, a model almost ubiquitously accepted in other fields of comparative biology, may be poorly suited to the study of conotruncal abnormalities in human disease. However, while the use of atrioventricular-specific molecular markers has allowed extensive characterization of the development of the atrium and ventricle, the lack of any bulbus-specific markers has meant that this region of the zebrafish heart is poorly characterized and quite possibly misunderstood. We have discovered that the fluorescent nitric oxide indicator 4,5-diaminofluorescein diacetate (DAF-2DA) specifically labels the bulbus arteriosus throughout development from approximately 48 It postfertilization. Therefore, using DAF-2DA and an immunohistochemical approach, we attempted to further characterize the development of the bulbus. We have concluded that no such phenotypic transition occurs, that contrary to current thinking, aspects of zebrafish arterial pole development are evolutionarily conserved, and that the bulbus should not be considered a chamber, being more akin to the arterial trunk(s) of higher vertebrates. (C) 2005 Elsevier Inc. All rights reserved.