Comparative Study of Anti-Oncogenic MicroRNA-145 in Canine and Human Malignant Melanoma
Comparative Study of Anti-Oncogenic MicroRNA-145 in Canine and Human Malignant Melanoma
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DOI：
10.1292/jvms.11-0264
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发表时间：
2012-01-01
影响因子：
1.2
通讯作者：
Maruo, Kohji
中科院分区：
文献类型：
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作者：
Noguchi, Shunsuke;Mori, Takashi;Maruo, Kohji
MicroRNA-145 （miRNA-145; miR-145）在大多数人类癌症中异常表达，在癌变和癌症进展中起重要作用。在目前的研究中，我们主要关注miR-145在犬和人类恶性黑色素瘤中的作用。MiR-145在犬恶性黑色素瘤组织和犬黑色素瘤细胞系以及人类黑色素瘤细胞系中均显著下调。miR-145的异位表达在犬和人黑色素瘤细胞中均显示出显著的生长抑制作用，其部分作用是通过抑制犬黑色素瘤LMeC和人黑色素瘤A2058和Mewo细胞中的c-MYC来实现的。同时，还发现犬黑色素瘤KMeC细胞有抑制细胞迁移的趋势，人类黑色素瘤A2058细胞通过抑制FASCIN1显著抑制细胞迁移。这些发现表明，miR-145在犬和人恶性黑色素瘤中都是肿瘤抑制因子。
MicroRNA-145 (miRNA-145; miR-145) is aberrantly expressed in most of human cancers and plays a significant role in carcinogenesis and cancer progression. In the current study, we focused on how miR-145 plays a role in canine and human malignant melanomas. MiR-145 was significantly downregulated in canine malignant melanoma tissues and canine melanoma cell lines, as well as human melanoma cell lines tested. The ectopic expression of miR-145 showed a significant growth inhibition in both canine and human melanoma cells tested, and the effect was achieved partly by suppressing c-MYC in canine melanoma LMeC and in human melanoma A2058 and Mewo cells. At the same time, a suppressive tendency on cell migration in canine melanoma KMeC cells and significant suppression of cell migration in human melanoma A2058 cells by suppressing FASCIN1 were also found. These findings suggest that miR-145 acts as a tumor suppressor in both canine and human malignant melanomas.