Snake venomics of central American pitvipers:: Clues for rationalizing the distinct envenomation profiles of Atropoides nummifer and Atropoides picadoi
Snake venomics of central American pitvipers:: Clues for rationalizing the distinct envenomation profiles of Atropoides nummifer and Atropoides picadoi
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DOI：
10.1021/pr700610z
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发表时间：
2008-02-01
影响因子：
4.4
通讯作者：
Calvete, Juan J.
中科院分区：
文献类型：
--
作者：
Angulo, Yamileth;Escolano, Jose;Calvete, Juan J.
我们报告的蛋白质组学特征的中美洲pitvipers Atropoides nummifer和Atropoides picadoi。粗毒液通过反相高效液相色谱法（HPLC）进行分级分离，然后通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SIDS-PAGE）、N-末端测序、基质辅助激光解吸电离飞行时间（MALDI-TOF）质量指纹和碰撞诱导解离串联质谱（CID-MS/MS）分析胰蛋白酶肽的每个色谱级分。每种毒液含有许多缓激肽增强肽和大约25-27种分子量在7-112 kDa范围内的蛋白质，属于仅9种不同的毒素家族（去整合素、DC片段、蛇毒血管内皮生长因子、磷脂酶A（2）、丝氨酸蛋白酶、富含半胱氨酸的分泌蛋白、C型凝集素、L-氨基酸氧化酶和Zn 2+依赖性金属蛋白酶），虽然明显分布在两个Atropoides物种。此外，A. nummifer表达少量的三指毒素，在A. picadoi。A. nummifer属于PLA（2）（相对丰度为36.5%）和丝氨酸蛋白酶（22%）家族，而A. Picadoi毒素是Zn ~（2+）依赖的金属蛋白酶（66.4%）。我们估计两个Atropoides类群之间的毒液蛋白的相似性可能在14- 16%左右。高度分化的毒液蛋白质组之间的同源类群强调独特的方面的毒液组成的相关物种的Atropoides蛇，并指出一个强大的作用，通过自然选择的适应性多样化作为这种独特性的原因。另一方面，其独特的毒液毒素组成提供了合理化的低出血，凝血，去凝血活性和高肌毒和蛋白水解作用引起的A。与其它响尾蛇蛇毒相比，银汉蛇毒具有较高的出血活性。皮卡多伊。
We report the proteomic characterization of the Central American pitvipers Atropoides nummifer and Atropoides picadoi. The crude venoms were fractionated by reverse-phase high-performance liquid chromatography (HPLC), followed by analysis of each chromatographic fraction by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SIDS-PAGE), N-terminal sequencing, matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass fingerprinting, and collision-induced dissociation-tandem mass spectrometry (CID-MS/MS) of tryptic peptides. Each venom contained a number of bradykinin-potentiating peptides and around 25-27 proteins of molecular masses in the range of 7-112 kDa, belonging to only nine different toxin families (disintegrin, DC fragment, snake venom vascular endothelial growth factor, phospholipases A(2), serine protease, cysteine-rich secretory proteins, C-type lectins, L-amino acid oxidase, and Zn2+-dependent metalloproteases), albeit distinctly distributed among the two Atropoides species. In addition, A. nummifer expresses low amounts of a three-finger toxin not detected in the venom of A. picadoi. The major toxins of A. nummifer belong to the PLA(2) (relative abundance, 36.5%) and the serine proteinase (22%) families, whereas the most abundant A. picadoi toxins are Zn2+-dependent metalloproteinases (66.4%). We estimate that the similarity of venom proteins between the two Atropoides taxa may be around 14-16%. The high degree of differentiation in the venom proteome among congeneric taxa emphasizes unique aspects of venom composition of related species of Atropoides snakes and points to a strong role for adaptive diversification via natural selection as a cause of this distinctiveness. On the other hand, their distinct venom toxin compositions provide clues for rationalizing the low hemorrhagic, coagulant, and defibrinating activities and the high myotoxic and proteolytic effects evoked by A. nummifer snakebite in comparison to other crotaline snake venoms and the high hemorrhagic activity of A. picadoi.