Role of adaptive and innate immune cells in chronic fatigue syndrome/myalgic encephalomyelitis
Role of adaptive and innate immune cells in chronic fatigue syndrome/myalgic encephalomyelitis
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DOI：
10.1093/intimm/dxt068
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发表时间：
2014-04-01
影响因子：
4.4
通讯作者：
Marshall-Gradisnik, Sonya M.
中科院分区：
文献类型：
--
作者：
Brenu, Ekua Weba;Huth, Teilah K.;Marshall-Gradisnik, Sonya M.
对 CFS/ME 中免疫细胞的全面分析。免疫过程的扰动是许多自身免疫性疾病和炎症性疾病的标志。慢性疲劳综合征/肌痛性脑脊髓炎 (CFS/ME) 是一种可能与自身免疫相关的炎症性疾病，其特征是 NK 细胞活性降低、调节性 T 细胞 (Treg) 升高和细胞因子水平失调。本文的目的是检查先前未在 CFS/ME 患者中检查过的先天性和适应性免疫细胞表型和功能特征。本研究招募了 30 名 CFS/ME 患者和 25 名非疲劳对照组。从所有参与者收集全血样本，用于使用流式细胞术方案评估细胞表型、功能特性、受体、粘附分子、抗原和细胞内蛋白质。研究的细胞包括 NK 细胞、树突状细胞、中性粒细胞、B 细胞、T 细胞、γ δ T 细胞和 Tregs。与非疲劳对照相比，CFS/ME 患者的 B 细胞亚群、Treg、CD4CD73CD39 T 细胞、细胞毒活性、颗粒酶 B、中性粒细胞抗原、TNF- 和 IFN- 均观察到显着变化。 B 细胞、Treg、NK 细胞和中性粒细胞的变化表明 CFS/ME 的免疫调节存在显着受损，这些可能与许多自身免疫性疾病有相似之处。
A comprehensive analysis of immune cells in CFS/ME.Perturbations in immune processes are a hallmark of a number of autoimmune and inflammatory disorders. Chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is an inflammatory disorder with possible autoimmune correlates, characterized by reduced NK cell activity, elevations in regulatory T cells (Tregs) and dysregulation in cytokine levels. The purpose of this article is to examine innate and adaptive immune cell phenotypes and functional characteristics that have not been previously examined in CFS/ME patients. Thirty patients with CFS/ME and 25 non-fatigued controls were recruited for this study. Whole blood samples were collected from all participants for the assessment of cell phenotypes, functional properties, receptors, adhesion molecules, antigens and intracellular proteins using flow cytometric protocols. The cells investigated included NK cells, dendritic cells, neutrophils, B cells, T cells, gamma delta T cells and Tregs. Significant changes were observed in B-cell subsets, Tregs, CD4CD73CD39 T cells, cytotoxic activity, granzyme B, neutrophil antigens, TNF- and IFN- in the CFS/ME patients in comparison with the non-fatigued controls. Alterations in B cells, Tregs, NK cells and neutrophils suggest significant impairments in immune regulation in CFS/ME and these may have similarities to a number of autoimmune disorders.