NFC-enabling smartphone-based portable amperometric immunosensor for hepatitis B virus detection
NFC-enabling smartphone-based portable amperometric immunosensor for hepatitis B virus detection
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DOI：
10.1016/j.snb.2020.128825
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发表时间：
2021-01-01
影响因子：
8.4
通讯作者：
Chailapakul, Orawon
中科院分区：
文献类型：
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作者：
Teengam, Prinjaporn;Siangproh, Weena;Chailapakul, Orawon
完全通过近场通信 (NFC) 操作的智能手机控制的电化学传感器被用来创建一种简单的无标记免疫测定法，用于检测乙型肝炎病毒 (HBV)。完整的系统由与智能手机集成的卡片大小的电化学 NFC 标签传感器和抗体修饰电极传感器组成。丝网印刷石墨烯电极 (SPGE) 用金纳米粒子进行修饰，提高了高灵敏度，同时在表面电聚合 β-环糊精 (β-CD) 以捕获抗体。通过电化学阻抗谱（EIS）和循环伏安法（CV）验证了修饰和固定化过程。然后，基于 NFC 的电化学免疫传感器用于通过电流检测，通过测量添加 HBsAg 之前和之后来自 (Fe(CN)(6))(3-/4-) 氧化还原对的电流来量化乙型肝炎表面抗原 (HBsAg)。 HBsAg 的线性校准曲线和检测限分别为 10-200 μg/mL 和 0.17 μg/mL。然后用慢性乙型肝炎病毒感染的血清样本对该传感器进行测试，该系统显示出与传统免疫测定良好的相关性。这种电化学免疫传感器有潜力成为便携式、简单、灵敏和选择性工具的替代平台，可以使用通过智能手机操作的低成本传感器系统轻松评估各种健康指标。
A smartphone-controlled electrochemical sensor operated entirely via Near Field Communication (NFC) was used to create a simple label-free immunoassay for detecting Hepatitis B Virus (HBV). The completed system was composed of a card-sized electrochemical NFC tag sensor integrated with a smartphone and antibody-modified electrode sensor. Screen-printed graphene electrodes (SPGE) were modified with gold nanoparticles, increasing high sensitivity while beta-cyclodextrin (beta-CD) was electropolymerized on the surface to capture antibodies. The modification and immobilization processes were verified by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The NFC-based electrochemical immunosensor was then used to quantify Hepatitis B surface antigen (HBsAg) using amperometric detection by measuring the current from the (Fe(CN)(6))(3-/4-) redox couple before and after addition of HBsAg. The linear calibration curve and limit of detection for HBsAg were found to be 10-200 mu g/mL and 0.17 mu g/mL, respectively. The sensor was then tested with chronic HBV infected serum samples and the system showed a good correlation with traditional immunoassays. This electrochemical immunosensor has the potential to be an alternative platform for portable, simple, sensitive and selective tools that can readily assess a variety of health indicators using a low-cost sensor system operated from a smartphone.