A fluorospectrophotometric study on the binding of acridine orange with DNA and its bases.
A fluorospectrophotometric study on the binding of acridine orange with DNA and its bases.
复制标题
吖啶橙与DNA及其碱基结合的荧光分光光度研究。
DOI：
10.1016/0003-9861(69)90020-4
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发表时间：
1969
影响因子：
3.9
通讯作者：
S. Yamabe
中科院分区：
文献类型：
--
作者：
S. Yamabe
为了证实吖啶橙子（AO）与DNA结合的性质，本文对AO-DNA体系、AO单核苷酸和AO-单核苷酸肽体系进行了荧光分光光度研究。在AO/DNA比值较高的（I）区，AO的荧光强度明显减弱，而在AO/DNA比值较低的（II）区，AO的荧光强度明显增强。观察到类似的变化与monoclonsides和monoclontides，虽然它们对荧光强度的影响很弱。有一个很好的平行之间的荧光光谱的变化和吸收光谱的变化已经报道。因此，确认了AO-DNA结合的两种机制，即在（I）区域中的自缔合和在（II）区域中的嵌入。比较了四种核苷对荧光强度的影响，发现A：T核苷对嵌入荧光增强的贡献大于G：C核苷对嵌入荧光增强的贡献。尿素、KCl和乙二胺对AO与DNA结合的逆转作用表明，在（I）区，AO与DNA的磷酸基团之间的离子键合占主导地位，而在（II）区，AO与DNA碱基之间的疏水键合占主导地位。对AO和肝素的典型异染体系进行了比较研究。
To confirm the nature of the binding of acridine orange (AO) with DNA, a fluorospectrophotometric study was made on the AO-DNA system as well as on the AOmononucleoside and AO-mononucleotide systems. In the (I) region of higher AO/DNA ratios, the fluorescence intensity of AO decreased markedly, while in the (II) region of lower AO/DNA ratios it increased markedly. Similar changes were observed with mononucleosides and mononucleotides, though their effects on fluorescence intensity were weak.There is a good parallel between these fluorospectral changes and the changes in the absorption spectrum already reported. Therefore, it was confirmed that there are two types of mechanism of AO-DNA binding, namely, self-association, in the (I) region and intercalation in the (II) region. Comparison of the effects of the four nucleosides upon the fluorescence intensity indicated that the contribution of an A:T nucleotide pair to the enhancement of fluorescence due to intercalation is more important than that of a G:C nucleotide pair. The reversing effects of urea, KC1, and ethylene diamine on AO-DNA binding indicate that in the (I) region, ionic bonding between AO and the phosphate groups of DNA is predominant, while in the (II) region, hydrophobic bonding between AO and the bases of DNA is predominant. A comparative study was made on a typical metachromatic system of AO and heparin.