Insect mitochondrial genomics 3: the complete mitochondrial genome sequences of representatives from two neuropteroid orders: a dobsonfly (order Megaloptera) and a giant lacewing and an owlfly (order Neuroptera)
Insect mitochondrial genomics 3: the complete mitochondrial genome sequences of representatives from two neuropteroid orders: a dobsonfly (order Megaloptera) and a giant lacewing and an owlfly (order Neuroptera)
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DOI：
10.1139/g08-098
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发表时间：
2009-01-01
期刊：
影响因子：
3.1
通讯作者：
Stewart, James Bruce
中科院分区：
文献类型：
--
作者：
Beckenbach, Andrew T.;Stewart, James Bruce
我们描述了完整的线粒体基因组的代表两个订单的脉翅目：一个dobsonfly，Corydalus cornutus（大翅目：Corydalidae，GenBank登录号FJ171323），一个巨大的草蛉Polystoechotes punctatus（脉翅目：Polystoechotidae，FJ171325），和一个猫头鹰，Ascaloptynx apericulatus（脉翅目：Ascalaphidae，FJ171324）。dobsonfly序列为15 687个碱基对，主要非编码区（富含A+ T）约967个碱基对。果蝇的基因内容和组织与大多数昆虫相同。大草蛉基因序列长16 036 bp，主要非编码区长1123 bp，而夜蛉基因序列长15 877 bp，主要非编码区长1066 bp。两个脉翅目序列包括两个tRNA基因的转座，tRNA（Trp）和tRNA（Cys）。这些tRNA基因在相反的链上编码，并在标准昆虫线粒体基因排列中重叠7个残基。因此，转座需要至少重叠区域的复制。转座很可能是通过两个基因的复制，然后每个基因的一个拷贝缺失而发生的。在其他两个neuropteroid物种，蛇蝇，Agulla sp.（针翅目：Raphidiidae），和一个antlion，Myringon immaculatus（Neuroptera：Myringontidae）的检查这一区域，表明重排是广泛的，在该命令Neuroptera，但不存在于Neuropterida的其他两个命令。
We describe the complete mitochondrial genomes from representatives of two orders of the Neuropterida: a dobsonfly, Corydalus cornutus (Megaloptera: Corydalidae, GenBank Accession No. FJ171323), a giant lacewing Polystoechotes punctatus (Neuroptera: Polystoechotidae, FJ171325), and an owlfly, Ascaloptynx appendiculatus (Neuroptera: Ascalaphidae, FJ171324). The dobsonfly sequence is 15 687 base pairs with a major noncoding (A+ T rich) region of approximately 967 bp. The gene content and organization of the dobsonfly is identical to that of most insects. The giant lacewing sequence is 16 036 bp with a major noncoding region of about 1123 bp, while the owlfly sequence is 15 877 bp with a major noncoding region of about 1066 bp. The two Neuroptera sequences include a transposition of two tRNA genes, tRNA(Trp) and tRNA(Cys). These tRNA genes are coded on opposite strands and overlap by seven residues in the standard insect mitochondrial gene arrangement. Thus, the transposition required a duplication of at least the region of overlap. It is likely that the transposition occurred by a duplication of both genes followed by deletion of one copy of each gene. Examination of this region in two other neuropteroid species, a snakefly, Agulla sp. (Raphidioptera: Raphidiidae), and an antlion, Myrmeleon immaculatus (Neuroptera: Myrmeleontidae), shows that the rearrangement is widespread in the order Neuroptera but not present in either of the other two orders of Neuropterida.