Antibacterial and antifungal effect of 405-nm monochromatic laser on endodontopathogenic microorganisms
Antibacterial and antifungal effect of 405-nm monochromatic laser on endodontopathogenic microorganisms
复制标题
405 nm 单色激光对牙髓病原微生物的抗菌和抗真菌作用
DOI：
10.1155/2014/387215
复制
发表时间：
2014
影响因子：
3.2
通讯作者：
Kazuhito Satomura
中科院分区：
文献类型：
--
作者：
Takahiro Imamura;Seiko Tatehara;Yusuke Takebe;Reiko Tokuyama; Tomoko Ohshima;Nobuko Maeda;Kazuhito Satomura
本研究的目的是评估 405 nm 单色激光照射作为预防和/或治疗牙髓感染的替代治疗方法的有效性。开发了配备 405 nm 激光二极管的单色激光发射装置。使用该装置，通过活菌落计数评估了 405 nm 激光照射对牙龈卟啉单胞菌、中间普雷沃氏菌、粪肠球菌和白色念珠菌（这些与持续性牙髓感染相关的微生物）生长的影响。结果表明，405nm激光照射对P． gingivalis, P. intermedia, andC. albicans, whereas the growth ofE. faecaliswas not affected by the irradiation. P 中的抑制率。牙龈和 P.在 0.2 W 照射 300 秒后，中间值分别为约 60% 和约 80%。抑制率C. 0.2 W 照射 1200 秒后，白色念珠菌的感染率约为 90%。这些结果表明405nm单色激光照射对这些微生物具有杀菌/杀真菌作用。本研究清楚地表明，405 nm 激光照射是预防和/或治疗牙髓感染的一种有前途的替代管理策略。
The purpose of this study is to evaluate the usefulness of 405 nm monochromatic laser irradiation as an alternative management for prevention and/or treatment of endodontic infections. A monochromatic laser‐emitting device equipped with a 405‐nm laser diode was developed. Using this device, the effect of 405 nm laser irradiation on the growth ofPorphyromonas gingivalis, Prevotella intermedia, Enterococcus faecalis, andCandida albicans, which are microorganisms associated with persistent endodontic infections, was evaluated by viable colony counting. As a result, the irradiation with a 405 nm laser had a significant bactericidal/fungicidal effect onP. gingivalis, P. intermedia, andC. albicans, whereas the growth ofE. faecaliswas not affected by the irradiation. The inhibition rate inP. gingivalisandP. intermediawas ~60% and ~80%, respectively, following irradiation at 0.2 W for 300 sec. The inhibition rate inC. albicanswas ~90% following irradiation at 0.2 W for 1200 sec. These results indicate that 405 nm monochromatic laser irradiation exerts a bactericidal/fungicidal effect on these microorganisms. The present study clearly demonstrates that 405 nm laser irradiation is a promising alternative management strategy for prevention and/or treatment of endodontic infections.