Enzyme-embedded electrospun fiber sensor of hydrophilic polymer for fluorometric ethanol gas imaging in vapor phase
Enzyme-embedded electrospun fiber sensor of hydrophilic polymer for fluorometric ethanol gas imaging in vapor phase
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DOI：
10.1016/j.bios.2022.114453
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发表时间：
2022-10-01
影响因子：
12.6
通讯作者：
Takeda, Naoya
中科院分区：
文献类型：
--
作者：
Iitani, Kenta;Nakaya, Misa;Takeda, Naoya
对生物体排放的挥发性有机化合物（VOC）的非侵入性测量是用于诊断人类健康状况或疾病的强大技术。生物基气体传感器适用于从复杂的VOC混合物中灵敏地选择性地测量目标VOC。传统的生物基传感器通常被制备为湿型探针，以保持蛋白质如酶处于稳定状态，导致传感器的商业化、其操作环境和性能受到限制。在这项研究中，我们提出了一种基于酶的荧光电纺纤维传感器（eFES）网作为气相生物传感器在干燥的形式。通过简单的一步电纺聚乙烯醇与醇脱氢酶和氧化形式的烟酰胺腺嘌呤二核苷酸制备靶向乙醇的eFES网。包埋在eFES网中的酶在干燥状态下不经预处理而活跃地工作。底物特异性也得到了保持，传感器对乙醇的响应良好，具有足够的动态范围。在eFES网中的pH和辅酶量的调节也影响酶活性。由于其优异的性能和简单的制作工艺，使其成为一种新型的气相生物传感器，有利于商品化。
Non-invasive measurement of volatile organic compounds (VOCs) emitted from living organisms is a powerful technique for diagnosing health conditions or diseases in humans. Bio-based gas sensors are suitable for the sensitive and selective measurement of a target VOC from a complex mixture of VOCs. Conventional bio-based sensors are normally prepared as wet-type probes to maintain proteins such as enzymes in a stable state, resulting in limitations in the commercialization of sensors, their operating environment, and performance. In this study, we present an enzyme-based fluorometric electmspun fiber sensor (eFES) mesh as a gas-phase biosensor in dry form. The eFES mesh targeting ethanol was fabricated by simple one-step electrospinning of polyvinyl alcohol with an alcohol dehydmgenase and an oxidized form of nicotinamide adenine dinucleotide. The enzyme embedded in the eFES mesh worked actively in a dry state without pretreatment. Substrate specificity was also maintained, and the sensor responded well to ethanol with a sufficient dynamic range. Adjustment of the pH and coenzyme quantity in the eFES mesh also affected enzyme activity. The dry-form biosensor-eFES mesh-will open a new direction for gas-phase biosensors because of its remarkable performance and simple fabrication, which is advantageous for commercialization.