RATIONAL DEVELOPMENT OF A SIMPLE SYNTHETIC MEDIUM FOR THE SUSTAINED GROWTH OF LACTOCOCCUS-LACTIS
RATIONAL DEVELOPMENT OF A SIMPLE SYNTHETIC MEDIUM FOR THE SUSTAINED GROWTH OF LACTOCOCCUS-LACTIS
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DOI：
10.1111/j.1365-2672.1995.tb03131.x
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发表时间：
1995-07-01
期刊：
影响因子：
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通讯作者：
LOUBIERE, P
中科院分区：
文献类型：
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作者：
COCAIGNBOUSQUET, M;GARRIGUES, C;LOUBIERE, P
两株乳酸乳球菌亚种的生长。在不同培养基上比较了蔬菜源（NCDO 2118）和乳制品源（IL 1403）的乳酸菌。这两种菌株在复杂的有机培养基上比在特定的合成培养基上生长得更快。在氮气相下生长效果最好。为了确定真正的营养需求，对非优化合成培养基的每个组分应用单遗漏技术。两种菌株对宏量元素、寡量元素、碱基和维生素的需要量相同。正如预期的那样，当使用单遗漏技术时，乳制品菌株（IL 2403）被认为对某些氨基酸具有营养缺陷，而蔬菜菌株（NCDO 2118）被认为对ah氨基酸具有营养缺陷。然后在逐渐简化的培养基上进行生长表征，并确定了两种菌株生长的绝对最小培养基的组成。蔬菜菌株只有在补充了六种氨基酸（Glu, Met, IIe, Leu, Val, Ser）的培养基中才能持续生长，这表明原生营养/营养不良的定义部分取决于培养基组成。除了蔬菜菌株生长所需的6种氨基酸外，乳制品菌株还需要Arg、His和Thr。从培养基中去除铵盐不影响生长，说明氨基酸可以满足生物质合成所需的全部氮。
The growth of two strains of Lactococcus lactis subsp. lactis from vegetable (NCDO 2118) and dairy origin (IL 1403) were compared on various culture media. Both strains grew more rapidly on a complex organic medium than on a defined synthetic medium. The best growth was obtained under nitrogen gas phase. The single omission technique was applied to each component of a non-optimized synthetic medium in order to determine the true nutritional requirements. Requirements for macro-elements, oligo-elements, bases and vitamins were identical for the two strains. As expected, the dairy strain (IL 2403) was seen to be auxotrophic for some amino acids, whereas the vegetable strain (NCDO 2118) was seen to be prototrophic for ah amino acids when using the single omission technique. Growth was then characterized on progressively simplified media and the composition of the absolute minimal media for the growth of both strains was defined. Sustained growth of the vegetable strain was only possible in minimal media supplemented with six amino acids (Glu, Met, IIe, Leu, Val, Ser), indicating that the definition of prototrophy/auxotrophy is partly dependent upon the medium composition. The dairy strain showed a requirement for Arg, His and Thr in addition to the six amino acids necessary for growth of the vegetable strain. The removal of ammonium salt from the medium did not affect the growth, illustrating that the amino acids may satisfy the totality of the nitrogen requirement for biomass synthesis.