Aging and accuracy of protein synthesis in man: search for inactive enzymatic cross-reacting material in granulocytes of aged people.
Aging and accuracy of protein synthesis in man: search for inactive enzymatic cross-reacting material in granulocytes of aged people.
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人类蛋白质合成的衰老和准确性：寻找老年人粒细胞中的无活性酶交叉反应物质。
DOI：
10.1159/000212156
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发表时间：
1976
期刊：
影响因子：
3.5
通讯作者：
J. Dreyfus
中科院分区：
文献类型：
--
作者：
H. Rubinson;A. Kahn;P. Boivin;F. Schapira;C. Grégori;J. Dreyfus
本文测定了10例新生儿、10例老年人(80岁以上)和10例青壮年(20-30岁)多形核白细胞中7种不同酶的活性和免疫反应性。之所以选择多形核细胞，是因为无论是在新生儿还是在老年人中，它们都是年轻的细胞，寿命只有几个小时。用单特异性抗酶血清对5种细胞质酶进行了免疫学研究：免疫灭活的白细胞丙酮酸激酶，电扩散法的葡萄糖-6-磷酸脱氢酶和葡萄糖-磷酸异构酶，放射免疫扩散法的乳酸脱氢酶(肌型)。用相同的多价抗人白细胞兔血清免疫滴定了两种溶酶体酶(α-甘露糖苷酶和β-葡萄糖苷酸酶)。观察到几种酶的活性存在微小但显著的差异。相比之下，在新生儿、年轻人和老年人之间，七种酶中的任何一种在酶活性/免疫反应性比率方面都没有发现显著差异。这些结果并不支持蛋白质合成机制的准确性降低是衰老和死亡的原因这一理论。相反，作者强调，不同作者在老年动物或古老的成纤维细胞培养中报告的酶异常与在人类葡萄糖-6-磷酸脱氢酶的情况下被证明是“翻译后修饰”的酶非常相似。
Enzyme activities and immunological reactivities of seven different enzymes have been assayed in the polymorphonuclear leukocytes from 10 newborns, 10 aged (over 80 years old) and 10 young adults (20-30 years old). Polymorphonuclears have been chosen because both in the newborns, and in the aged people, they are young cells whose life span is a few hours only. Five cytoplasmic enzymes have been immunologically studied by means of monospecific antienzyme sera: leukocyte pyruvate kinase by immunoinactivation, glucose-6-phosphate dehydrogenase and glucose phosphate-isomerase by electroimmunodiffusion, lactic dehydrogenase (muscle-type) by radial immunodiffusion. Two lysosomal enzymes (alpha-mannosidase and beta-glycuronidase) have been titrated immunologically with the same polyvalent antihuman leukocyte rabbit serum. Small but significant differences in activity were observed for several of the enzymes. By contrast, no significant difference was found, for any of the seven enzymes, as regards the ratio enzyme activity/immunological reactivity, between newborns, young adults and aged people. These results of not support the theory that a decreased accuracy of the protein synthesis machinery is responsible for aging and death. By contrast, the authors emphasize that the enzyme abnormalities reported by various authors in old animals or in old fibroblast cultures closely resemble those demonstrated to be "post-translational modifications" in the case of human glucose-6-phosphate dehydrogenase.