Timing and process of entry of zooxanthellae into oocytes of hermatypic corals
Timing and process of entry of zooxanthellae into oocytes of hermatypic corals
复制标题
虫黄藻进入造血珊瑚卵母细胞的时间和过程
作者：
M. Hirose;R. Kinzie;M. Hidaka
抽象的。我们研究的时间和机制进入卵母细胞的虫黄藻在四个物种的石珊瑚产卵含有这些共生藻类。脉单孔珊瑚Montipora venosa、M.在产卵前7-10 d采集指状棘孔珊瑚、疣状棘孔珊瑚和艾杜氏棘孔珊瑚的样品，进行光镜和电镜观察。在所有四个物种中，卵母细胞被薄的中胶层和外卵泡层包裹。单孔珊瑚和泡孔珊瑚卵中虫黄藻的数量和分布不同。产卵前5-7天，虫黄藻首先在卵泡细胞中出现。产卵前几天，卵母细胞和中胶层之间的细胞过程消失了。从卵泡细胞中排出的虫黄质通过中胶层的临时间隙，可能通过吞噬作用进入卵母细胞。我们的研究结果表明，卵泡细胞介导的祖黄嘌呤从父母卵母细胞的转移。
Abstract. We studied the timing and mechanism of entry of zooxanthellae into oocytes in four species of scleractinian corals that spawn eggs containing these symbiotic algae. Gonads of Montipora venosa, M. digitata, Pocillopora verrucosa, and P. eydouxi were sampled for 7–10 days before spawning and examined with light and electron microscopes. In all four species, oocytes were enveloped by a thin mesogleal layer and an outer follicle layer. The number and distribution of zooxanthellae in spawned eggs differed between Montipora and Pocillopora. Zooxanthellae were first observed in the follicle cells 5–7 days before spawning. A few days before spawning, the cellular processes that had intervened between oocytes and mesoglea disappeared. Zooxanthellae that were expelled from the follicle cells passed through temporary gaps in the mesoglea and probably entered the oocytes by phagocytosis. Our results suggest that follicle cells mediate the transfer of zooxanthellae from the parent to oocytes.