Response of extracelluar zinc in the ventral hippocampus against novelty stress
Response of extracelluar zinc in the ventral hippocampus against novelty stress
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DOI：
10.1111/j.1471-4159.2006.04092.x
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发表时间：
2006-10
影响因子：
4.7
通讯作者：
A. Takeda;Naomi Sakurada;S. Kanno;A. Minami;N. Oku
中科院分区：
文献类型：
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作者：
A. Takeda;Naomi Sakurada;S. Kanno;A. Minami;N. Oku
在探索行为中，海马体中发生了广泛的神经元活动。然而，海马体锌在探索行为中的作用尚不清楚。为了分析海马细胞外锌对新应激的反应，将大鼠置于新环境中50 min，每天1次，连续8天。在第1天的探索行为中，海马的细胞外谷氨酸增加，而细胞外锌减少。在第2天的探索行为中观察到同样的现象，并且在第8天的探索行为中细胞外锌已恢复到基础水平。为了检验细胞外锌在探索活动中减少的意义，在灌注1mm CaEDTA（一种膜不渗透锌螯合剂）时观察了探索行为。CaEDTA灌注降低了新环境下的运动活动。灌流CaEDTA可消除探索期细胞外锌含量下降和细胞外谷氨酸含量升高的现象。这些结果表明，海马细胞对锌的摄取与探索活动有关，并且是激活谷氨酸能神经递质系统所必需的。锌的摄取可能与无痛心理应激反应或认知过程有关。
An extensive neuronal activity takes place in the hippocampus during exploratory behavior. However, the role of hippocampal zinc in exploratory behavior is poorly understood. To analyze the response of extracellular zinc in the hippocampus against novelty stress, rats were placed for 50 min in a novel environment once a day for 8 days. Extracellular glutamate in the hippocampus was increased during exploratory behavior on day 1, whereas extracellular zinc was decreased. The same phenomenon was observed during exploratory behavior on day 2 and extracellular zinc had returned to the basal level during exploratory behavior on day 8. To examine the significance of the decrease in extracellular zinc in exploratory activity, exploratory behavior was observed during perfusion with 1 mm CaEDTA, a membrane‐impermeable zinc chelator. Locomotor activity in the novel environment was decreased by perfusion with CaEDTA. The decrease in extracellular zinc and the increase in extracellular glutamate in exploratory period were abolished by perfusion with CaEDTA. These results suggest that zinc uptake by hippocampal cells is linked to exploratory activity and is required for the activation of the glutamatergic neurotransmitter system. The zinc uptake may be involved in the response to painless psychological stress or in the cognitive processes.