Field-transcriptome analyses reveal developmental transitions during flowering in cassava (Manihot esculenta Crantz)
Field-transcriptome analyses reveal developmental transitions during flowering in cassava (Manihot esculenta Crantz)
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DOI：
10.1007/s11103-021-01149-5
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发表时间：
2021-04-15
影响因子：
5.1
通讯作者：
Tsuji, Hiroyuki
中科院分区：
文献类型：
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作者：
Behnam, Babak;Higo, Asuka;Tsuji, Hiroyuki
关键信息我们描述了在木薯光周期开花途径中起作用的基因。转录组分析显示这些基因的特征表达模式，表明田间种植木薯经历两个不同的发展转变。木薯是一种重要的食用和工业作物。木薯生长出储存淀粉的根，为热带地区提供了重要的主食来源。为了促进木薯育种，重要的是阐明开花是如何控制的。控制开花时间的几个重要基因已经在模式植物中被确定，然而，这些基因在木薯中的全面表征仍然缺乏。在这项研究中，我们确定了基因编码的中央开花时间调节和检查这些序列的存在或不存在的保守基序。我们发现，木薯共享光周期开花途径的保守基因，包括成花素、抗成花素及其相关转录因子（GIGANTEA、CONSTANS、FLOWRENLOCUS T、CENTRORADIALIS/MATERIAL FLOWER 1和FD）和成花素下游基因（SUPEROR OF OVEREXPRESSION OF CONSTANS 1和APETALA 1/FRUITFUL）。我们对田间生长的木薯植物进行了RNA-seq分析，并表征了开花控制基因的表达。最后，从转录组分析，我们确定了两个不同的发展转变，发生在田间种植木薯。
Key message We characterized genes that function in the photoperiodic flowering pathway in cassava. Transcriptome analysis of field-grown plants revealed characteristic expression patterns of these genes, demonstrating that field-grown cassava experiences two distinct developmental transitions. Cassava is an important crop for both edible and industrial purposes. Cassava develops storage roots that accumulate starch, providing an important source of staple food in tropical regions. To facilitate cassava breeding, it is important to elucidate how flowering is controlled. Several important genes that control flowering time have been identified in model plants; however, comprehensive characterization of these genes in cassava is still lacking. In this study, we identified genes encoding central flowering time regulators and examined these sequences for the presence or absence of conserved motifs. We found that cassava shares conserved genes for the photoperiodic flowering pathway, including florigen, anti-florigen and its associated transcription factor (GIGANTEA, CONSTANS, FLOWERING LOCUS T, CENTRORADIALIS/TERMINAL FLOWER1 and FD) and florigen downstream genes (SUPRESSOR OF OVEREXPRESSION OF CONSTANS1 and APETALA1/FRUITFUL). We conducted RNA-seq analysis of field-grown cassava plants and characterized the expression of flowering control genes. Finally, from the transcriptome analysis we identified two distinct developmental transitions that occur in field-grown cassava.