Vax genes ventralize the embryonic eye
Vax genes ventralize the embryonic eye
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DOI：
10.1101/gad.1276605
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发表时间：
2005-05-15
影响因子：
10.5
通讯作者：
Bertuzzi, S
中科院分区：
文献类型：
--
作者：
Mui, SH;Kim, JW;Bertuzzi, S
脊椎动物的视网膜和视神经在大小、组织和细胞多样性方面有着惊人的不同，但这两种结构都是从同一个胚胎神经上皮细胞发育而来的。前体细胞在最腹侧域的上皮只产生视神经的星形胶质细胞，而紧邻，更背侧的前体细胞产生无数的细胞类型的视网膜。我们提供的遗传学证据表明，两个密切相关的，腹侧表达的同源结构域蛋白Vax1和Vax2控制这种神经上皮分离。在缺乏这两种蛋白质的情况下，我们发现视神经完全转化为完全分化的视网膜。我们证明，这种转变的结果从开发的眼领域的腹侧活性的损失，腹侧介导的，至少部分地，通过Vax抑制的Pax6基因，一个有效的诱导剂的视网膜发育。
The vertebrate retina and optic nerve are strikingly different in terms of their size, organization, and cellular diversity, yet these two structures develop from the same embryonic neuroepithelium. Precursor cells in the most ventral domain of this epithelium give rise only to the astrocytes of the optic nerve, whereas immediately adjacent, more dorsal precursors give rise to the myriad cell types of the retina. We provide genetic evidence that two closely related, ventrally expressed homeodomain proteins-Vax1 and Vax2-control this neuroepithelial segregation. In the absence of both proteins, we find that the optic nerve is transformed in its entirety into fully differentiated retina. We demonstrate that this transformation results from the loss of ventralizing actvity in the developing eye field, and that ventralization is mediated, at least in part, via Vax repression of the Pax6 gene, a potent inducer of retinal development.