Biocompatibility and Cytotoxic Evaluation of New Sorbent Cartridges for Blood Hemoperfusion
Biocompatibility and Cytotoxic Evaluation of New Sorbent Cartridges for Blood Hemoperfusion
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DOI：
10.1159/000489921
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发表时间：
2018-01-01
影响因子：
3
通讯作者：
Ronco, Claudio
中科院分区：
文献类型：
--
作者：
Montin, Diego Pomare;Ankawi, Ghada;Ronco, Claudio
背景/目的：血液灌流（HP）吸附盒的使用正在迅速发展。对于这些器械，潜在的诱导细胞毒性是一个重要问题。本研究的目的是研究不同吸附剂滤芯（HA 130、HA 230、HA 330、HA 380（Jafron，中国））对U937单核细胞的潜在体外细胞毒性作用。方法：以静态和动态方式将单核细胞暴露于吸附材料。在静态试验中，在检测盒中孵育24 h后采集细胞培养基样品。在动态试验中，进行了HP模态，并在30、60、90和120 min采集样品。结果如下：与对照样本相比，在静态和动态试验中，暴露于测试卡片的单核细胞中没有坏死或凋亡增加的证据。结论：我们的体外试验表明，HA钉仓具有最佳水平的生物相容性，其在HP中的使用与不良反应或细胞毒性迹象无关。(C)2018年S. Karger AG，巴塞尔。
Background/Aims: The use of adsorption cartridges for hemoperfusion (HP) is rapidly evolving. For these devices, the potential induced cytotoxicity is an important issue. The aim of this study was to investigate potential in vitro cytotoxic effects of different sorbent cartridges, HA130, HA230, HA330, HA380 (Jafron, China), on U937 monocytes. Methods:Monocytes were exposed to the sorbent material in static and dynamic manners. In static test, cell medium samples were collected after 24 h of incubation in the cartridges. In dynamic test, HP modality has been carried out and samples at 30, 60, 90, and 120 min were collected. Results: Compared to control samples, there was no evidence of increased necrosis or apoptosis in monocytes exposed to the cartridges both in the static and dynamic tests. Conclusion: Our in vitro testing suggests that HA cartridges carry an optimal level of biocompatibility and their use in HP is not associated with adverse reactions Or Signs Of CytotoXicity. (C) 2018 S. Karger AG, Basel.