CLONING AND EXPRESSION OF MURINE INTERLEUKIN-1 CDNA IN ESCHERICHIA-COLI
CLONING AND EXPRESSION OF MURINE INTERLEUKIN-1 CDNA IN ESCHERICHIA-COLI
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DOI：
10.1038/312458a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
MIZEL, SB
中科院分区：
文献类型：
--
作者：
LOMEDICO, PT;GUBLER, U;MIZEL, SB
白细胞介素-1（IL-1）是一种由活化的巨噬细胞产生的肽类激素，具有调节多种细胞类型增殖、成熟和功能活化的能力1 - 7，并可能通过其对这些不同细胞群的作用在免疫和炎症反应的启动和放大中发挥重要作用8。IL-1在其相对分子质量（Mr，13，000 - 19，000）和电荷性质8方面表现出微观异质性，尽管小鼠IL-1已被纯化9，10，并且其一些基本结构-功能关系已被阐明8，但已证明难以制备足够量的IL-1用于直接和详细的序列和结构研究。本文报道了小鼠IL-1cDNA的克隆、序列分析及其在大肠杆菌中的表达。IL-1 cDNA编码270个氨基酸的多肽前体。在E.大肠杆菌表达IL-1前体的羧基末端156个氨基酸。
Interleukin-1 (IL-1), a peptide hormone produced by activated macrophages, possesses the ability to modulate the proliferation, maturation and functional activation of a broad spectrum of cell types1–7and may play a major role in the initiation and amplification of immune and inflammatory responses through its action on these diverse cell populations8. IL-1 exhibits microheterogeneity in terms of its relative molecular mass (Mr, 13,000–19,000) and charge properties8, and although murine IL-1 has been purified9,10and some of its basic structure–function relationships have been elucidated8, it has proved difficult to prepare sufficient amounts of IL-1 for direct and detailed sequence and structural studies. Here we report the cloning, sequence analysis and expression of murine IL-1 cDNA inEscherichia coli. The IL-1 cDNA codes for a polypeptide precursor of 270 amino acids. Biologically active IL-1 was produced inE. coliby expressing the carboxy-terminal 156 amino acids of the IL-1 precursor.