THE O2 GENE WHICH REGULATES ZEIN DEPOSITION IN MAIZE ENDOSPERM ENCODES A PROTEIN WITH STRUCTURAL HOMOLOGIES TO TRANSCRIPTIONAL ACTIVATORS
THE O2 GENE WHICH REGULATES ZEIN DEPOSITION IN MAIZE ENDOSPERM ENCODES A PROTEIN WITH STRUCTURAL HOMOLOGIES TO TRANSCRIPTIONAL ACTIVATORS
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DOI：
10.1002/j.1460-2075.1989.tb08425.x
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发表时间：
1989-10-01
期刊：
影响因子：
11.4
通讯作者：
THOMPSON, R
中科院分区：
文献类型：
--
作者：
HARTINGS, H;MADDALONI, M;THOMPSON, R
玉米醇溶蛋白调节基因Opaque 2的结构已通过基因组和cDNA克隆的序列分析确定。O2 mRNA大小为1751 bp（不包括poly（A）尾），包含一个1380 bp的开放阅读框（ORF），前有3个短的开放阅读框，分别为3、21和20个氨基酸残基。该基因的开放阅读框长1362 bp，由6个外显子和5个内含子组成，外显子大小为465 ~ 61 bp，内含子大小为678 ~ 83 bp。一个推定的蛋白质454个氨基酸长的理论翻译的基因组序列对应的外显子。opaque 2蛋白含有与动物原癌基因如fos、jun和myc的DNA结合蛋白以及转录激活因子GCN 4和C/EBP中鉴定的亮氨酸拉链基序相似的结构域。靠近N末端亮氨酸重复序列的30个氨基酸残基的区域富含碱性氨基酸，并且也与fos、jun和GCN 4中存在的结构域同源。此外，在羧基末端区域中存在与金属结合结构域非常相似的氨基酸基序。
The structure of the zein regulatory gene Opaque 2 of Zea mays has been determined by sequence analysis of genomic and cDNA clones. The size of O2 mRNA is 1751 bp [poly(A) tail not included] containing a major open reading frame (ORF) of 1380 bp preceded by three short ORFs of 3, 21 and 20 amino acid residues. The main ORF comprises 1362 bp and is composed of six exons ranging in size from 465 to 61 bp and five introns of 678 bp to 83 bp. A putative protein 454 amino acids long was derived by the theoretical translation of the genomic sequences corresponding to exons. The opaque 2 protein contains a domain similar to the leucine zipper motif identified in DNA binding proteins of animal protooncogenes such as fos, jun and myc, and in the transcriptional activators GCN4 and C/EBP. The region of 30 amino acid residues next to the leucine repeats towards the N terminus is rich in basic amino acids and is also homologous to a domain present in fos, jun and GCN4. Moreover, in the carboxy terminal region an amino acid motif closely resembling a metal binding domain is present.