Presence of immunoreactive salusin-α in human serum and urine
Presence of immunoreactive salusin-α in human serum and urine
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DOI：
10.1016/j.peptides.2006.06.005
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发表时间：
2006-11-01
期刊：
影响因子：
3
通讯作者：
Shichiri, Masayoshi
中科院分区：
文献类型：
--
作者：
Sato, Kengo;Koyama, Takatoshi;Shichiri, Masayoshi
Salusins是通过生物信息学分析从全长丰富的人类cDNA文库中鉴定出来的，在大鼠身上显示出促有丝分裂、神经调节和血流动力学活性。它们在各种各样的人体组织中表达，但它们在人体体液中的确切结构和水平仍不清楚。我们建立了一种适合于检测免疫反应性人Salusin-α的放射免疫分析方法，并对人血清和尿液中Salusin-a的分子形式和浓度进行了表征。用辛基硅胶柱提取血清后，可测定低至1fmol/管的免疫活性Salusin-a浓度，结合抑制50%所需浓度为40fmol/管。与Salusin-beta和其他生物活性多肽的交叉反应可以忽略不计。正常人血清和尿液中Salusin-α免疫反应性分别为11.0~40.4pmoL/L(平均值+/-SD，23.3+/-8.1pmol/L，n=31)和18.6~367.3 pmol/L(平均值+/-S.D.，156.8+/-95.8pmol/L)。反相高效液相色谱结合放射免疫分析检测显示，一个主要的免疫活性成分，与正品Salusin-a洗脱。这些数据表明在人的血清和尿液中存在salusin-a，从而验证了最初预测的salusin-a在人类中的加工位点。(C)2006 Elsevier Inc.保留所有权利。
Salusins, identified from a full-length enriched human cDNA library by bioinformatics analyses, show mitogenic, neuromodulatory and hemodynamic activities in rats. They are expressed in a wide variety of human tissues, but their precise structures and levels in human body fluids remain unknown. We developed a radioimmunoassay suitable for the detection of immunoreactive human salusin-alpha and characterized the molecular forms and concentrations of salusin-a in human serum and urine. The assay allowed for measurement of immunoreactive salusin-a concentrations as low as 1 fmol/tube after extraction of serum with an octyl-silica column, and the concentration required for 50% inhibition of binding was 40 fmol/tube. Cross-reactivities with salusin-beta and other bioactive peptides were negligible. Salusin-alpha-like immunoreactivity in normal human serum and urine ranged from 11.0 to 40.4 pmol/l (mean +/- S.D., 23.3 +/- 8.1 pmol/l, n = 31) and from 18.6 to 367.3 pmol/l (mean +/- S.D., 156.8 +/- 95.8 pmol/l), respectively. Reverse-phase high performance liquid chromatography coupled with radioimmunoassay detection revealed a major immunoreactive component that coeluted with authentic salusin-a. These data indicate the presence of salusin-a in human serum and urine, thereby verifying the initially predicted processing sites for salusin-a in humans. (c) 2006 Elsevier Inc. All rights reserved.