THE P250 SUBUNIT OF NATIVE TATA BOX-BINDING FACTOR-TFIID IS THE CELL-CYCLE REGULATORY PROTEIN-CCG1
THE P250 SUBUNIT OF NATIVE TATA BOX-BINDING FACTOR-TFIID IS THE CELL-CYCLE REGULATORY PROTEIN-CCG1
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DOI：
10.1038/362179a0
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发表时间：
1993-03-11
期刊：
影响因子：
64.8
通讯作者：
ROEDER, RG
中科院分区：
文献类型：
--
作者：
HISATAKE, K;HASEGAWA, S;ROEDER, RG
TFIID蛋白是一种通用转录因子1，通过与TATA启动子元件直接相互作用启动前起始复合体组装2 -4 5，6。它是一种多亚基复合物，含有一个小的TATA结合多肽（TBP）和其他TBP相关因子（TAF），大小约为30- 250 K（参考文献7-10）。虽然天然TFIID可以介导重建系统中的激活子非依赖性（基础）和激活子依赖性转录3，5，6，但TBP本身只能介导基础转录11，12，即使在已知TBP或一般因子TFIIB直接与转录激活子相互作用的情况下13 -15。因此，TFIID亚基而不是TBP必须是必需的辅因子，因此是激活剂的潜在靶点，这与早期证明激活剂与TFIID相互作用一致（参考文献3，5，16，17）。在这里，我们表明TFIID的250 K亚基与先前参与细胞周期晚期G1期进展的基因产物相同18，19。因此，p250的一部分可能在激活对细胞周期进程重要的基因子集中发挥特定功能。
THE protein TFIID is a general transcription factor1 which initiates preinitiation complex assembly2-4 through direct interaction with the TATA promoter element5,6. It is a multisubunit complex containing a small TATA-binding polypeptide (TBP) and other TBP-associated factors (TAFs) ranging in size from about 30-250K (refs 7-10). Although native TFIID can mediate both activator-independent (basal) and activator-dependent transcription in reconstituted systems3,5,6, TBP itself can mediate only basal transcription11,12, even in cases where TBP or the general factor TFIIB are known to interact directly with transcriptional activators13-15. TFIID subunits other than TBP must therefore be essential cofactors, and thus potential targets for activators, consistent with earlier demonstrations that activators interact with TFIID (refs 3, 5, 16, 17). Here we show that the 250K subunit of TFIID is identical to a gene product previously implicated in progression through the late G1 phase of the cell cycle18,19. Part of p250 may thus serve a specific function in the activation of a subset of genes important for cell cycle progression.