Characterization and phylogenomics of the complete mitochondrial genome of the polyzoic cestode Gangesia oligonchis (Platyhelminthes: Onchoproteocephalidea)
Characterization and phylogenomics of the complete mitochondrial genome of the polyzoic cestode Gangesia oligonchis (Platyhelminthes: Onchoproteocephalidea)
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多生代绦虫 Gangesia olivenchis（扁形动物：Onchoproteocephalidea）完整线粒体基因组的表征和系统发育学
DOI：
10.1017/s0022149x19000452
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发表时间：
2020
影响因子：
1.6
通讯作者：
G.T.Wang
中科院分区：
文献类型：
--
作者：
W.X.Li;D.Zhang;P.P.Fu;R.Song;H.Zou;M.Li;S.G.Wu;G.T.Wang
原足目是最近建立起来的，以容纳带有钩的四足目和蛋白头足目，其特征是内部先兆化和四髋臼头节。基于28S重组DNA （rDNA）序列数据，鉴定出的蛋白头科亚科为非单系亚科。显然还需要其他具有更高系统发育分辨率的分子标记，如大的线粒体DNA片段和多基因。因此，Gangesia oligonchis（被认为是protecephalidae中最早的分化群）的线粒体基因组被测序。少毛鸡圆形有丝分裂基因组大小为13958 bp，包含36个标准基因：22个转移RNA基因、2个rRNA基因和12个蛋白质编码基因，以及2个主要的非编码区。短NCR和大NCR （lNCR）区大小分别为216 bp和419 bp。在lNCR区检测到11个重复单元的高重复区。G. oligonchis有丝分裂基因组与testdotaenia sp. WL-2016核苷酸同源性为71.1%。利用贝叶斯推断和最大似然方法对全线粒体基因组进行系统发育分析，结果表明，G. oligonchis与testdotaenia sp. WL-2016形成了最大支持度的姐妹进化支。序拓扑结构为（石竹科）、（双叶竹科）、（双叶竹科）、（Onchoproteocephalidea、Cyclophyllidea）)))。G. oligonchis有丝分裂基因组基因排列与testdotaenia sp. WL-2016相同。有丝分裂基因组学和核序列数据需要更多的分类群来有效地探索Onchoproteocephalidea之间的相互关系。
The order Onchoproteocephalidea (Eucestoda) was recently erected to accommodate the hook-bearing tetraphyllideans and the proteocephalideans, which are characterized by internal proglottization and a tetra-acetabulate scolex. The recognized subfamilies in the Proteocephalidae appeared to be non-monophyletic based on 28S recombinant DNA (rDNA) sequence data. Other molecular markers with higher phylogenetic resolution, such as large mitochondrial DNA fragments and multiple genes, are obviously needed. Thus the mitochondrial genome of Gangesia oligonchis, belonging to the putative earliest diverging group of the Proteocephalidae, was sequenced. The circular mitogenome of G. oligonchis was 13,958 bp in size, and contained the standard 36 genes: 22 transfer RNA genes, two rRNA genes and 12 protein-coding genes, as well as two major non-coding regions. A short NCR and a large NCR (lNCR) region were 216 bp and 419 bp in size, respectively. Highly repetitive regions in the lNCR region were detected with that of 11 repeat units. The mitogenome of G. oligonchis shared 71.1% nucleotide identity with Testudotaenia sp. WL-2016. Phylogenetic analyses of the complete mitochondrial genomes with Bayesian inference and maximum likelihood methods indicated that G. oligonchis formed a sister clade with Testudotaenia sp. WL-2016 with maximum support. The ordinal topology is (Caryophyllidea, (Diphyllobothriidea, (Bothriocephalidea, (Onchoproteocephalidea, Cyclophyllidea)))). The mitogenomic gene arrangement of G. oligonchis was identical to that of Testudotaenia sp. WL-2016. Both mitogenomic and nuclear sequence data for many more taxa are required to effectively explore the inter-relationships among the Onchoproteocephalidea.