Infection of the ferret stomach by isogenic flagellar mutant strains of Helicobacter mustelae.
Infection of the ferret stomach by isogenic flagellar mutant strains of Helicobacter mustelae.
复制标题
雪貂胃被鼬螺杆菌同基因鞭毛突变株感染。
DOI：
10.1128/iai.65.5.1962-1966.1997
复制
发表时间：
1997
影响因子：
3.1
通讯作者：
Suerbaum,S
中科院分区：
文献类型：
--
作者：
Andrutis,KA;Fox,JG;Schauer,DB;Marini,RP;Li,X;Yan,L;Josenhans,C;Suerbaum,S
鼬螺杆菌与幽门螺杆菌一样，具有两种鞭毛蛋白FlaA和FlaB。H.已经通过用卡那霉素抗性盒破坏flaA或flaB基因或通过引入卡那霉素和氯霉素抗性基因以产生双突变体来构建鼬。为了确定是否一个或两个鞭毛蛋白是必要的定植和持续感染H。白鼬19只，无H.给鼬施用HMF 1 flaA：：km（弱运动型）、ATCC 43772 flaB：：km（中等运动型）或HMF 1 flaA：：cat flaB：：km（非运动型）突变株、野生型亲本株或无菌肉汤。从接种后3周开始，到接种后3个月尸检结束，在连续的胃活检过程中获得胃组织样本。H.通过培养、组织学和血清学确定鼬感染状态。H.鼬在所有时间点感染所有雪貂。双突变菌株无法定殖; flaA和flaB单突变菌株最初能够以低水平定殖，并随着时间的推移建立持续感染，生物体数量不断增加。这些H.鼬与胃粘膜中存在的微生物数量相关。鞭毛运动是一个重要的毒力因子的定居和致病的H。鼬貂模型。
Helicobacter mustelae, like Helicobacter pylori, possesses two flagellin proteins, FlaA and FlaB. Isogenic mutant strains of H. mustelae have been constructed by disruption of the flaA or flaB gene with a kanamycin resistance cassette or by introduction of both a kanamycin and a chloramphenicol resistance gene to produce a double mutant. To determine whether one or both flagellin proteins are necessary for colonization and persistence of infection with H. mustelae, 19 ferrets, specific pathogen free for H. mustelae, were given either the HMF1 flaA::km (weakly motile), ATCC 43772 flaB::km (moderately motile), or HMF1 flaA::cat flaB::km (non-motile) mutant strain, the wild-type parent strains, or sterile broth. Gastric tissue samples were obtained during sequential gastric biopsies beginning at 3 weeks postinoculation and ending at necropsy at 3 months postinoculation. H. mustelae infection status was determined by culture, histology, and serology. The wild-type parent strains of H. mustelae infected all ferrets at all time points. The double-mutant strain was unable to colonize; the flaA and flaB single-mutant strains were able to initially colonize at a low level and establish persistent infection with increasing numbers of organisms over time. The severity of gastritis produced by infection with these strains of H. mustelae correlated with the number of organisms present in the gastric mucosa. Flagellar motility is an important virulence factor for colonization and pathogenesis in the H. mustelae ferret model.