SOMATIC MUTATION CREATES DIVERSITY IN THE MAJOR GROUP OF MOUSE IMMUNOGLOBULIN KAPPA-LIGHT CHAINS
SOMATIC MUTATION CREATES DIVERSITY IN THE MAJOR GROUP OF MOUSE IMMUNOGLOBULIN KAPPA-LIGHT CHAINS
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DOI：
10.1084/jem.159.2.417
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发表时间：
1984-01-01
影响因子：
15.3
通讯作者：
TONEGAWA, S
中科院分区：
文献类型：
--
作者：
HEINRICH, G;TRAUNECKER, A;TONEGAWA, S
利用克隆的小鼠Ig .kappa的cDNA[互补DNA]。L链在骨髓瘤MOPC321 （V .kappa）中的合成。-21亚群C)作为13种生殖系V.KAPPA的探针。可以检测到基因片段。其中11例是分离的。一组重叠的克隆片段被用来证明这些种系中的9个。基因排列在2个连锁簇中，并且它们都具有相同的转录取向（11,12,22）。这两个簇分别占染色体DNA的90 kb和30 kb[千碱基]，含有6个和3个V.kappa。,分别。5种种系V.kappa的全核苷酸序列。结果表明，其中3个编码了V.kappa的原型序列。-21亚群B、C和e。编码已知V.kappa的变异氨基酸序列。-21子组。就像。lambda。1L链，变异V区显然是由相应种系DNA的一些体细胞突变衍生的基因片段编码的。这种体细胞突变不限于编码高变区的序列；它们也出现在编码框架区域的序列中。
Using a cloned cDNA [complementary DNA] of a mouse Ig .kappa. L chain synthesized in a myeloma MOPC321 (V .kappa.-21 subgroup C) as a probe 13 germ line V.KAPPA. gene segments could be detected. Of these 11 were isolated. A set of overlapping cloned segments was used to show that 9 of these germ line V.kappa. genes are arranged in 2 linkage clusters and that they all have the same transcriptional orientation (11, 12, 22). These 2 clusters occupy 90 and 30 kb [Kilobase] of chromosomal DNA and contain 6 and 3 V.kappa., respectively. The complete nucleotide sequences of 5 germ line V.kappa. were determined and it was that 3 of them encode the prototype sequence of V.kappa.-21 subgroups B, C and E. None of these 5 germ line V.kappa. encodes the variant amino acid sequences of known V.kappa.-21 subgroups. As in the .lambda.1 L chains, the variant V regions are apparently encoded by gene segments derived by a few somatic mutations from the corresponding germ line DNA. Such somatic mutations are not restricted to sequences encoding the hypervariable regions; they also occur in sequences encoding framework regions.