FORMATION OF HEPARAN-SULFATE OR CHONDROITIN XDERMATAN SULFATE ON RECOMBINANT DOMAIN-I OF MOUSE PERLECAN EXPRESSED IN CHINESE-HAMSTER OVARY CELLS
FORMATION OF HEPARAN-SULFATE OR CHONDROITIN XDERMATAN SULFATE ON RECOMBINANT DOMAIN-I OF MOUSE PERLECAN EXPRESSED IN CHINESE-HAMSTER OVARY CELLS
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DOI：
10.1006/bbrc.1995.1805
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发表时间：
1995-06-06
影响因子：
3.1
通讯作者：
SILBERT, JE
中科院分区：
文献类型：
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作者：
KOKENYESI, R;SILBERT, JE
小鼠串珠素的重组结构域I在中国仓鼠卵巢（CHO K1）细胞中表达，并在Ni-琼脂糖上亲和纯化。凝胶色谱法，随后通过使用糖胺聚糖裂解酶表征糖胺聚糖显示，重组蛋白聚糖平均含有三条硫酸乙酰肝素或软骨素/硫酸皮肤素的糖胺聚糖链，中值大小约为12 kDa。这些数据表明，结构域I具有用于连接糖胺聚糖的功能位点，并表明天然串珠素的糖胺聚糖链在其N-末端分组。这反过来又表明，串珠蛋白聚糖中结构域I的可能功能是向核心蛋白添加糖胺聚糖链。(C)出版社：Academic Press
Recombinant domain I of mouse perlecan was expressed in Chinese hamster ovary (CHO K1) cells and affinity purified on Ni-agarose, Gel chromatography followed by characterization of glycosaminoglycans by the use of glycosaminoglycan lyases showed that the recombinant proteoglycans contained, on average, three glycosaminoglycan chains of heparan sulfate or chondroitin/dermatan sulfate of approximately 12 kDa median size. These data demonstrate that domain I has functional sites for attachment of glycosaminoglycans and indicate that the glycosaminoglycan chains of native perlecan are grouped at its N-terminal end. This, in turn, suggests that the likely function of domain I in perlecan would be to provide for the addition of glycosaminoglycan chains to the core protein. (C) 1995 Academic Press, Inc.